水稻半矮化多分蘖突变体f2-132的表型分析和基因定位  

Phenotypic Analysis and Gene Mapping of the Semi-Dwarf High-Tillering Mutant f2-132 in Rice

作　　者：张艳培[1,2] 孙伟 尹亮 赵金凤[2] 袁守江 于元杰[1] 李学勇[2] 

机构地区：[1]山东农业大学农学院,泰安271018 [2]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081 [3]山东省水稻研究所,济南250100

出　　处：《植物遗传资源学报》2014年第1期165-170,共6页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31271311)

摘　　要：半矮化多分蘖突变体f2-132由60Co-γ辐射诱变粳稻品种F2-285A获得。遗传分析表明该性状受1对隐性基因控制,已将突变基因定位在第4染色体长臂上2个Indel标记C4-Z3和C4-Z4之间,物理距离为46 kb。该区间内包含一个已报道的多分蘖基因D17/HTD1,对f2-132中的D17基因测序发现编码区第395位的碱基由T突变为C,导致第132位的氨基酸由苯丙氨酸变成丝氨酸。D17/HTD1编码类胡萝卜素裂解双加氧酶7(CCD7,carotenoid cleavage dioxygenase 7),是植物激素独脚金内酯(SLs,strigolactones)合成途径中的重要酶之一。利用SLs的人工合成类似物GR24处理f2-132,其多分蘖表型受到抑制。The high-tillering dwarf mutant f2-132 was obtained from the Japonica rice variety F2-285A radiated by ^60Co -y-ray. Genetic analysis indicated that this trait was controlled by one single recessive gene, which was mapped between two InDel markers C4-Z3 and C4-Z4 on the long arm of chromosome 4. The physical distance between the two markers was 46 kb,which contained a reported high-tillering dwarf gene D17/HTD1. Sequence analysis of the D17 allele inf2-132 showed that the base T at the 395th position in the coding region was substituted by C,which changed the 132th amino acid from phenylalanine to serine. D17/HTD1 encoded the carotenoid cleavage dioxygenase 7 （CCDT）, which was one of the key enzymes in the biosynthesis pathway of a new plant hormone, strigolactones （SLs）. After treatment with GR24, a synthetic analogues of SLs, the tillering phenotype was suppressed.

关 键 词：水稻 矮化多分蘖 类胡萝卜素裂解双加氧酶 独脚金内酯 

分 类 号：S511[农业科学—作物学]