平欧杂种榛主栽品种(系)遗传关系的ISSR分析  

Analysis of the Genetic Relationship for the Main Cultivars / Varieties of Ping'ou Hybrid Hazelnut by ISSR Markers

作　　者：陈新[1] 马庆华[2] 王贵禧[2] 赵天田[2] 贾建云[1] 徐丽[1] 张力思[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点试验室,泰安271000 [2]中国林业科学研究院林业研究所/林木遗传育种国家重点试验室/国家林业局林木培育试验室,北京100091

出　　处：《植物遗传资源学报》2014年第1期201-205,共5页Journal of Plant Genetic Resources

基　　金：山东省农业良种工程项目(鲁科农字:2011-086);国家林业局林业公益性行业科研专项(201304710);中央级公益性科研院所基本科研业务费专项资金(RIF2012-11);泰安市科技特派员项目(2012-15)

摘　　要：采用ISSR分子标记的方法，对达维、辽榛3号等17个平欧杂种榛主栽品种（系）进行了遗传关系研究。试验从60条ISSR引物中筛选出7条进行电泳分析，使用NTSYSpc 2．11F和Popgene 1．32软件进行数据统计分析并作图。结果显示：7条ISSR引物共获得58条谱带，平均每条引物8．3条谱带，引物平均多态性比率84．6％；7条ISSR引物可将所有样品完全分开，当遗传相似性阈值为0．687时可将样品分成3个类群：第1类群14个品种（系）可分成4个亚类，第Ⅱ类群1个品种，第Ⅲ类群2个品系。样品间的遗传相似性系数为0．448～0．879（平均0．678）。群体的有效等位基因数、基因多样度、Shannon信息指数分别为1．6751、0．3701和0．5308。上述结果表明，平欧杂种榛是一个具有高度遗传多样性的群体，主栽品种（系）间存在复杂的亲缘关系。该研究结果对于平欧杂种榛的遗传关系研究具有重要意义，也可为其他榛属植物的相关研究提供参考。The objective of this study is to analyze the genetic relationship for the main cultivars/varieties for Ping＇ ou hybrid hazelnut. 17 main cultivars/varieties, such as ＇ Dawei＇ and＇ Liaozhen No. 3＇,are selected as materials. 7 ISSR primers with high polymorphism, strong signals,and clear bands are selected from 60 primers. NTSYS pc 2.11F and Popgene 1.32 software are used to conduct the data analysis and plotting. Totally 58 bands are obtained. The average number of bands is 8.3 per primer and the average percentage of polymorphic bands is 84.6%. All the samples are divided into 3 groups at the similarity coefficient of O. 687. The first group, containing 14 cultivars/varieties, can be divided into four sub-groups whereas the second and the third group contain 1 and 2 cultivars, respectively. The similarity coefficient of the samples varies from 0. 448 to 0. 879, with an average of 0. 678. The effective number of alleles, Nei＇s gene diversity, and Shannon＇s information index of the main cultivars are 1. 6751,0. 3701 ,and 0. 5308, respectively. Ping＇ou hybrid hazelnut is a high polymorphic population and the genetic relationships of the main cultivars are complex. The result of this study can offer reference for the relative researches in Corylus.

关 键 词：平欧杂种榛 亲缘关系 遗传多样性 ISSR 

分 类 号：S664.4[农业科学—果树学]