辣椒花器官发育PAP3基因和PDS基因VIGS载体的构建及鉴定  

Construction and Identification of Flowering-related PAP3 Gene and PDS Gene VIGA Vector in Pepper

作　　者：马宁[1] 刘辰[1] 王茹芳[1] 沈火林[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国瓜菜》2014年第1期10-12,24,共4页China Cucurbits And Vegetables

基　　金：国家自然科学基金资助项目(项目批准号:31071806);现代农业产业体系北京市果类蔬菜创新团队项目

摘　　要：以辣椒恢复系121C为材料,采用RT-PCR技术从121C花药中克隆得到控制花器官发育B类基因PAP3的部分序列,片段长379 bp,阳性对照八氢番茄红素脱氢酶基因(phytoene desaturae,PDS),片段长323 bp,然后通过酶切分别连接pTRV2质粒,获得重组载体pTRV2-pPAP3和pTRV2-PDS。利用PCR进行初步鉴定后进行序列测定。结果表明,辣椒PAP3基因和PDS基因已插入到pTRV2载体上,VIGS载体构建成功。该研究为下一步分析基因沉默和辣椒花器官的发育提供了试验基础。In order to study the efficiency of gene-silencing in pepper floral organ development,part of the sequence frag- ment,length 379 bp, and positive control eight hydrogen lycopene dehydrogenase gene （phytoene desaturae, PDS）fragment, length 323 bp,were obtained from the anther of pepper restore line 121C as materials via semi-quantitative RT-PCR. Con- necting two genes individually to the pTRV2 plasmid,vector pTRV2-pPAP3 and pTRV2-PDS were restructured. After the preliminary identification with the help of PCR, the full construct was sequenced for confirmation. The result showed that PDS and PA P3 genes were inserted into the pTRV2 carrier. This study provided the test materials for further analysis in gene-si- lencing of pepper floral organ development and created a new approach for pepper male sterility breeding.

关 键 词：辣椒 PAP3 病毒诱导基因沉默 花器官发育 

分 类 号：S641.3[农业科学—蔬菜学]