小柴胡汤对糖皮质激素受体及其mRNA的调节作用  

Regulation of glucocorticoid receptor and its mRNA by Tsiao Shihutang

作　　者：杨霞[1] 王浩丹[2] 侯桂华[2] 王维岳 刘德宜[2] 赵敬杰[2] 

机构地区：[1]山东医科大学附属医院核医学科,山东济南250012 [2]山东医科大学同位素中心实验室,山东济南250012

出　　处：《中国病理生理杂志》2000年第12期1304-1306,共3页Chinese Journal of Pathophysiology

摘　　要：目的和方法 :给予健康雄性小鼠 (Balb/C)小柴胡汤 (TSS) ,糖皮质激素 (GC) ,应用受体放射配体结合分析法测定小鼠脾细胞糖皮质激素受体 (GR)位点数 ,同时用定量逆转录 多聚酶链反应 (RT PCR)法测定脾细胞内GRmRNA水平 ,以期从分子受体水平和基因水平探讨TSS对GR及GRmRNA的调节作用。结果和结论 :(1)应用TSS后GR位点数及GRmRNA水平明显少于正常对照组 ,说明TSS对GR的降调是通过调节GRmRNA水平实现的 ;(2 )TSS与GC合用时GR位点数及GRmRNA水平明显高于单独应用GC组 ,说明TSS可明显减弱GC对GR的降调作用。AIM: In order to explore the mechanism of the GC-like effect of Tsiao Shaihutang (TSS), the down-regulation of glucocorticoid receptor and mRNA by TSS were studied.METHODS: GR sites were determined by receptor radio ligand binding assay method. At the same time, GR mRNA level was determined by quantitative reverse-transcriptive polymerase chain reaction (RT-PCR) method.RESULTS: (1) GR sites and GR mRNA level were down-regulated significantly after GC, TSS (P<0.01) treatment . (2) GR sites and GR mRNA level in GC plus TSS group were obviously higher than those in GC group (P<0.01).CONCLUSIONS: These data suggested that TSS can significantly down-regulate GR and GR mRNA level.

关 键 词：糖皮质激素受体 MRNA TSS 小柴胡汤 小鼠 

分 类 号：R285.5[医药卫生—中药学]