丹参酮ⅡA对压力负荷增加大鼠心肌NF-κB表达的影响  

Effect of tanshinone Ⅱ A on expression of myocardial NF-κB in rats with pressure overload

作　　者：蔡辉[1] 蔡佳宇[1] 常文静[1] 赵智明[1] 董晓蕾[1] 赵凌杰[1] 

机构地区：[1]南京军区南京总医院中西医结合科,南京市210002

出　　处：《河北医药》2014年第2期180-183,共4页Hebei Medical Journal

基　　金：南京军区南京总医院科研基金面上课题(编号:2010Q027)

摘　　要：目的观察丹参酮ⅡA(TanshinoneⅡA,TanⅡA)对压力负荷增加大鼠心肌组织核因子-κB(NF-κB)表达的影响,并探讨其对心肌纤维化的保护作用。方法采用腹主动脉缩窄术制备压力负荷心肌纤维化模型,术后存活32只大鼠随机分成4组(n=8),假手术组、模型组、TanⅡA组(20 mg·kg-1·d-1)及阳性对照药物卡托普利组(100 mg·kg-1·d-1)。术后4周成功造模并开始给药,疗程为4周。8周后检测左心室重量指数、心肌组织病理学、心肌羟脯氨酸含量以及心肌组织NF-κB p65蛋白含量。结果与假手术组比较,模型组大鼠心室治疗指数和左心室质量指数明显升高(P<0.01);与模型组比较,TanⅡA则显著降低(P<0.01)。与假手术组比较,模型组大鼠心肌羟脯氨酸含量明显升高(P<0.01);与模型组比较,TanⅡA组则显著降低(P<0.01)。与假手术组比较,模型组大鼠心肌组织NF-κB p65表达显著升高(P<0.01);与模型组比较,TanⅡA组则显著降低(P<0.01)。结论 TanⅡA能抑制心肌肥厚,减轻心肌纤维化,此作用可能与部分下调NF-κB表达有关。Objective To observe the effect of tanshinone Ⅱ A （Tan ⅡA） on expression of myocardial NF-κB in rats with pressure overload, and to explore its protective effects on myocardial fibrosis. Methods The pressure overload myocardial fibrosis models were established by abdominal aorta constriction in Sprague-Dawley （ SD ） rats, these rats were randomly divided into four groups ： sham - operation group, model group, Tan 11 A group （ 20mg ·kg- 1 . d - 1 ） and positive control group （captopril 100mg·kg-1 . d-1） ,with 8 rats in each group. 4 weeks after the operation, the animal models were successfully established, then the rats were given drugs, with a course of 4 weeks. After 8 weeks, left ventricular mass index, myocardial tissue pathological changes,myocardial hydroxyproline content,myocardial NF-κB p65 expression were detected in rats of the four groups. Results As compared with those in sham - operation group, cardiac mass index and left ventricular mass index were significantly increased in model group （ P 〈 0. 01 ） ; as compared with those in model group, these indexes were obviously decreased in Tan II A group （ P 〈 0. O1 ）. As compared with that in sham - operation group, myocardial hydroxyproline content was significantly increased in model group （ P 〈 0.01 ）, however, as compared with that in model group,myocardial hydroxyproline content was obviously decreased in Tan ⅡA group （ P 〈0.01 ）. As compared with those in sham -operation group, NF-κB p65 protein expression levels in myocardial tissues were significantly increased in model group （ P 〈0.01） ,however,as compared with those in model group,which were significantly decreased in Tan Ⅱ A group （ P 〈0. 01 ）. Conclusion Tan II A can inhibit cardiac hypertrophy, relieve myocardial fibrosis, and the effect may be related to down - regulation of the expression of NF-κB.

关 键 词：丹参酮ⅡA 压力负荷 心肌纤维化 核因子-ΚB 

分 类 号：R542.23[医药卫生—心血管疾病]