LyP-1肽的^(131)碘标记及其生物分布研究  

The Study of ^(131)I Labeling and Biodistribution of LyP-1 Peptide

作　　者：陈月华 于明明[2] 郑瑞强 

机构地区：[1]扬州苏北人民医院ICU,江苏省扬州市225001 [2]青岛医学院附属医院PET-CT中心

出　　处：《医学理论与实践》2014年第1期1-3,8,共4页The Journal of Medical Theory and Practice

摘　　要：目的:探讨Na131I标记LyP-1肽的可能性以及Na131I标记的LyP-1肽在乳腺癌裸鼠体内的生物分布。方法:包含LyP-1序列的十环肽(YCGNKRTRGC)通过固相合成法合成。在两个半胱氨酸之间形成二硫键以维持肽的环形结构。LyP-1肽通过氯胺-T法进行Na131I标记。通过尾静脉途径将Na131I标记LyP-1肽以及对照肽注射于荷MDA-MB-435乳腺癌裸鼠体内并测量其生物分布情况。结果:LyP-1肽的Na131I标记率可达80%±5%(n=5),放射化学纯度约96%。在37℃新鲜人血清中Na131I标记LyP-1肽24h其放射化学纯度仍然达92%。在生物分布研究中,Na131I标记LyP-1肽在肿瘤中的聚集水平明显高于其他组织。结论:通过氯胺-T法可以成功的将131I标记于LyP-1肽。131I标记的LyP-1肽在37℃新鲜人血清中24h仍然保持稳定,并可被肿瘤组织高度摄取。Objective ：To observe the possibility of 131 I labeled LyP-1 peptide and its metastasis .Methods ：The 10-mer cyclic peptide contained the LyP-1 sequence （YCGNKRTRGC） was synthesized through the solid phase method .Disul-fide bonds between the cysteines maintain the cyclic structure .The LyP-1 peptide was labeled by 131 I using the chlora-mine-T measure .Control peptide and 131 I labeled LyP-1 peptide were injected into the nude mice bearing MDA-MB-435 xenografts via tail vein .Biodistribution in vivo was obtained .Results：The labeling efficiencies of LyP-1 peptide could reach 80% ± 5% （n= 5） .The radiochemical purity reached 96% .The radiochemical purity of the labeled compound maintained 92% in human blood serum at 37℃ at 24h .In the biodistribution studies ,the 131 I labeled LyP-1 peptide ac-cumulated in the tumor to a higher level than in the other organs .Conclusion：The LyP-1 peptide could be labeled with 131 I effectively and the labeled compound was stable at 24h in human blood serum at 37℃ and was evidently absorbed by tumor tissue .

关 键 词：LyP_1肽MDA-MB-435乳腺癌生物分布 

分 类 号：R817[医药卫生—影像医学与核医学]