超声造影监测丹参治疗兔肾缺血再灌注损伤的实验研究  

Experimental study on ultrasound contrast for monitoring Salvia miltiorrhiza in treating rabbit renal ischemia-reperfusion injury

作　　者：叶帆[1] 李明星[1] 罗志建[1] 张敏[1] 宣吉晴[1] 陈晓梅[1] 

机构地区：[1]泸州医学院附属医院超声诊断科,四川泸州646000

出　　处：《重庆医学》2014年第2期200-202,共3页Chongqing medicine

基　　金：四川省教育厅科研资助课题(08zc054)

摘　　要：目的运用超声造影定量分析技术监测丹参治疗兔肾缺血再灌注损伤前后肾皮质血流灌注的变化,探讨超声造影在评价药物治疗肾缺血再灌注损伤疗效中的应用价值。方法家兔切除右肾,夹闭左肾肾蒂缺血60min后恢复灌注建立兔左肾缺血再灌注损伤模型,并给予丹参注射液对比观察左肾病理改变及在超声造影下肾皮质血流灌注并应用时间-强度曲线定量分析肾皮质血流灌注参数。结果缺血再灌注损伤后,兔肾皮质时间-强度曲线上升缓慢,峰值灌注时间延长,曲线上升支斜率下降(P<0.05);经丹参治疗后峰值灌注明显缩短,曲线上升支斜率明显上升(P<0.05)。结论超声造影定量分析技术能反映兔肾缺血再灌注损伤所致的肾血流灌注异常,并能发现经药物治疗后兔肾血流再灌注的改变。Objective To apply the real-time gray scale contrast-enhanced ultrasonography（CEUS） with quantitative analysis technique to monitor the change of the renal cortex blood perfusion before and after Salvia miltiorrhiza treatment of rabbit renal is- chemia-reperfusion injury and to investigate the application value of the ultrasound contrast for evaluating the effect of medication in the treatment of rabbit renal ischemia-reperfusion injury（IRI）. Methods The rabbit right kidney was resected and the left renal pedicle was occluded for 60 min ischemia,then the perfusion was recovered for establishing the IRI model. Salvia miltiorrhiza injec- tion was given for observing the pathological changes of the left kidney and the renal cortex blood prefusion under the contrast-en- hanced ultrasonography. The parameters of renal cortex blood perfusion were quantitatively analyzed by the time-intension curves. Results After IRI occurrence, the time-intensity curve（TIC） of the rabbit renal cortex was slowly ascended. The time to peak （TTP） was extened and the rising slope （Grad） of the curve ascending branch was declined （P〈 0. 05 ） ; after Salvia miltiorrhiza treatment,TTP decreased and Grad increased obviously（P〈0.05）. Conclusion CEUS combined with quantitative analysis can re- flect the renal blood perfusion abnormity caused by ischemia-reperfusion and discover the change of the renal blood perfusion after medication treatment.

关 键 词：肾 缺血 再灌注损伤 灰阶超声造影 定量分析 丹参 兔 

分 类 号：R445.1[医药卫生—影像医学与核医学]