两种木霉生防菌对松落针病病原菌抑制作用研究  

Inhibition effect of two Trichoderma spp. on the pathogenic fungi of needle-cast of Pinus densiflora

作　　者：祁金玉[1] 高国平[1] 王一[1] 梁冬雪 张云川 孟庆娇 

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110866 [2]辽宁省生态公益林管理中心,辽宁沈阳110036 [3]辽宁省生态实验林场,辽宁朝阳122000 [4]辽宁省本溪市桓仁县林业局,辽宁本溪117201

出　　处：《中国森林病虫》2014年第1期14-16,共3页Forest Pest and Disease

基　　金：沈阳农业大学校青年基金(20121001)

摘　　要：通过绿色木霉Trichoderma viride、哈茨木霉T.harzianum对松落针病不同病原菌平板对峙培养及两种木霉粗提液对松落针病病原菌菌丝生长抑制试验研究表明:72 h时,哈茨木霉对针叶树散斑壳的抑制率为42.86%,对光亮散斑壳的抑制率为48.44%,绿色木霉对小环绵盘菌的抑制率为33.33%;两种木霉粗提液混合培养基法的试验效果均好于平板涂抹法;两种木霉粗提液对病原菌抑制效果最好的为绿色木霉的混合培养基法,对针叶树散斑壳Lophodermiurn conigenum、光亮散斑壳L.nitens、小环绵盘菌Cyclaneusula minus的抑菌率分别为64.3%,55.42%,61.44%。The experiments of antagonism of two Trichoderma. spp. （ Trichoderma viride and T. harzia-hum） against the pathogenic fungi of needle-cast of Pinus densiflora and the growth-inhibition effects of the crude extracts of the two Trichoderma on the above fungi were made. The results showed that in 72 hours,the inhibition rates of T. harzianum were 42. 86% on Lophodermium conigenum,48.44% on L. nitens,and that of T. viride on Cyclaneusula minus was 33.33%. The inhibiting effects of the mixed medium method of the two Trichoderma crude extracts were better than that of plate method. And the inhibiting effect of the mixed medium method of T. viride crude extract was the best： its inhibiting effect was 64. 3% on L. conigenum,55.42% on L. nitens and 61.44% on C. minus.

关 键 词：松落针病 木霉菌 拮抗作用 生物防治 

分 类 号：S763.15[农业科学—森林保护学]