6种治疗类风湿关节炎有效中药成分或组分整合对佐剂性关节炎大鼠血清及滑膜TNF-α、IL-1β作用的研究  

Study on the effect of the mixture of six active ingredients from herbs treating rheumatoid arthritis on the levels of TNF-α and IL-1β in serum and synovium by adjuvant arthritis rat model

作　　者：徐丽萍[1] 宋欣伟[1] 陈亚慧[2] 陈祺轩 关天容[1] 戴巧定[1] 张彦[1] 

机构地区：[1]浙江中医药大学附属第一医院风湿免疫科,杭州310006 [2]浙江中医药大学第一临床医学院,杭州310053

出　　处：《中华中医药杂志》2014年第1期249-252,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金项目(No.Y205677)~~

摘　　要：目的:探讨6种治疗类风湿关节炎(RA)有效的中药成分或组分整合对佐剂性关节炎(AA)大鼠血清及滑膜TNF-α、IL-1β的作用。方法:本实验共450只Wistar大鼠,随机预留20只为空白组,另430只参与制备AA大鼠模型。将造模成功的324只AA大鼠,按随机数字表法将其分成18组:第1-16组为中药整合组,按L16(215)正交计划表将6种中药有效成分进行整合后给药;第17组为雷公藤多苷组,予雷公藤多苷片溶液(12mg·kg-1·d-1),第18组为模型组,与空白组均灌胃给予等容量0.9%氯化钠溶液。分别在给药前及给药后第4、8周检测血清及滑膜肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)含量,经正交极差分析得出最佳整合方案,并评估其疗效。结果:各中药整合组经正交分析后得出了最佳整合方案,与模型组、雷公藤多苷组比较,能明显降低血清及滑膜TNF-α、IL-1β含量(P<0.05,P<0.01)。结论:多种治疗RA有效中药成分整合在治疗AA方面具有明显优势。Objective： Selected the best optimal integrated solution of six kinds of effective ingredients from traditional Chinese medicines for the treatment of rheumatoid arthritis （RA）, according to the L16（2^15） orthogonal table experiment and compared it to the Triperygium wilfordii multiglucoside （MT） in order to evaluate the anti-inflammatory effect. Methods： In this experiment, a total of 450 Wistar rats, reserved 20 as blank group at random, another 430 were used to establish the model of adjuvant rodents rheumatism arthritis （AA）. Divided the successful 324 AA rats into 18 groups according to random number table method. Group 1-16 were integration for traditional Chinese medicine groups, which were given the mixture with different formulas of effective medical ingredients from six traditional Chinese medicines by stomach irrigation according to the L16 （2^15） orthogonal analysis. Meanwhile, group 17, as MT group, was given MT （12mg.kg-1.d-1）. Group 18 as model group and blank group were given 0.9% sodium chloride solution by gavage. On the drug treatment of 0, 4, 8 weeks, detected TNF-α and IL-1 β in serum and joint synovial organizations （immunohistochemical）. Selected the best integrated solution by the orthogonal analysis and evaluated the efficacy. Results： According to the data from experiment, we got the best integration solution. Compared it to the model group, MT group and others, it was better upon reducing TNF-α and IL-1βin serum and joint synovial organizations （P〈0.05, P〈0.01）. Conclusion： Mixture of multiple traditional Chinese medicine effective components display the advantage in the therapy of rheumatoid arthritis, both in serology and histology.

关 键 词：类风湿关节炎 佐剂性关节炎 有效中药成分整合 肿瘤坏死因子-α 白细胞介素-1 β 

分 类 号：R285.5[医药卫生—中药学]