逍遥散对兔膝关节软骨细胞凋亡线粒体通路的影响  

Influence of Xiaoyao Powder on rabbit's knee cartilage tissue gene apoptosis mitochondrial pathway

作　　者：戴七一[1] 黎强[2] 郑铁牛[1] 覃学流[1] 林宗汉[1] 阮萍[1] 谈驰[1] 罗胤[2] 高辉[1] 冯彦江[1] 王甜[1] 詹楚宁[1] 王永乐[1] 

机构地区：[1]广西中医药大学附属瑞康医院,南宁530011 [2]广西中医药大学基础医学院,南宁530001

出　　处：《中华中医药杂志》2014年第1期282-285,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西科技厅自然基金资助项目(No.2012GXNSFAA053097)~~

摘　　要：目的:探讨逍遥散降低雌性兔膝关节软骨细胞凋亡的可能作用机制。方法:40只新西兰兔通过手术造成膝关节失稳型模型,1周后随机分为中药组、西药组、混合组和对照组各10只,驱赶行走4周,分别给药8周。造模后12周末处理动物,免疫组化法检测兔膝关节软骨细胞凋亡线粒体通路相关基因的表达。结果:中药组、西药组和混合组Bcl-2表达均显著高于对照组;Bax、Caspase-3与细胞色素的C表达均显著低于对照组。结论:逍遥散可明显上调Bcl-2表达,下调Bax、Caspase-3与细胞色素的C的表达,可能是逍遥散降低兔膝关节软骨细胞凋亡的机制之一。Objective： To explore the possible mechanism of Xiaoyao Powder on reducing female rabbit knee articular cartilage apoptosis cell. Method： 40 New Zealand rabbits were modeled through operation for knee joint instability, a week later, they were divided randomly into four groups： traditional Chinese medicine group, western medicine group, mixed group and control group, 10 in each group. Each rabbit was t＇orced to walk for 4 weeks, then was given medicine or saline perfusion by oral gavage for 8 weeks. 12 weeks later, the animals were executed, and the knee cartilage tissue apoptosis gene expressions were detected by immunohistochemical method. Results： The apoptosis expression Bcl-2 of traditional Chinese medicine group, western medicine group and mixed group were higher than control group, and the expression of Bax, Caspase-3 and Cytochrome C were lower, and there was significant difference. There was no significant difference among traditional Chinese medicine group, western medicine group and mixed group. Conclusion： The Xiaoyao Powder could decrease knee articular cartilage gene apoptosis expression of Bax, Caspase-3 and Cytochrome C and increase expression of Bcl-2, and this might be a possible mechanism of Xiaoyao Powder in reducing knee articular cartilage apoptosis cells in rabbits.

关 键 词：中医药疗法 骨关节炎 兔 凋亡基因 

分 类 号：R285.5[医药卫生—中药学]