基底样乳腺癌与管腔A乳腺癌干/祖细胞的表型分析  

Phenotypic analysis of stem/progenitor cells derived from basal-like and luminal-A breast cancer

作　　者：陈元文[1] 吴诚义[2] 董华英[2] 

机构地区：[1]重庆市第五人民医院普外科,400062 [2]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《检验医学与临床》2014年第2期165-167,共3页Laboratory Medicine and Clinic

摘　　要：目的比较人基底样乳腺癌和管腔A乳腺癌干/祖细胞分化前后的细胞表型变化。方法应用乳腺癌细胞球无血清悬浮培养法获取乳腺癌干/祖细胞;分别以荧光免疫技术和流式细胞术检测基底样乳腺癌和管腔A乳腺癌干/祖细胞分化前后细胞角蛋白CK14、CK18、CK19和CD44、CD24的表达情况;分析原代细胞内CD44+CD24-细胞水平与克隆形成率的关系。结果基底样乳腺癌干/祖细胞表达CK19+/CK14+,不表达CK18-,经血清诱导分化后,管腔上皮标志CK18和肌上皮标志CK14呈阳性表达;管腔A乳腺癌干/祖细胞呈CK18+/CK19+/CK14-表型,诱导分化后管腔上皮标志CK18和CK19表达,而肌上皮细胞标志CK14则不表达;CD24在基底样乳腺癌表达极低或无表达,但在管腔A乳腺癌高表达。基底样乳腺癌原代细胞中水平较高的是CD44-/CD24-/Low细胞,含CD44+/CD24-/Low细胞(2.52±0.58)%,而管腔A乳腺癌CD44-/CD24+细胞所占的比例最高,但不含CD44+/CD24-/Low细胞或水平极低。结论基底样乳腺癌和管腔A乳腺癌干/祖细胞有不同的细胞表型,二者的分化能力和分化方向也不同,可能起源于不同的乳腺上皮细胞。Objective To compare the phenotypic change of stern/progenitor cells from basal-like breast cancer and luminal-a breast cancer before and after differentiation. Methods The breast cancer stem/progenitor cells were obtained by the breast cancer mammosphere（MS） serum free Maitland culture; the expression of CK14, CK18, CK19,CD44 and CD24 before stem/progenitor cells differentiation in basal-like breast cancer and luminal-a breast cancer were detected by the immunofluorescence and the flow cytometry;the relation berween the content of CD44, CD24 cells in the primary cells with the cloning efficiency（Msfe） were analyzed. Results The stem/progenitor cells of basal-like breast cancer showed the CK19＋ /CK14＋ CK18 phenotype,after the induced differentiation,expressed the luminal epithelium marker CK18 and myoepithelium marker CK14. The stem/progenitor cell phenotype of basal- like breast cancer and luminal a breast cancer showed CK18＋/CK19＋/CK14 , after the induced differentiation,ex- pressed the luminal epithelium marker CK18 and CK19,did not express CK14 ;CD24 had the extreme low expression or no expression in basal-like breast cancer, but had high expression in luminal a breast cancer. CD44-/CD24-/Low ceils had hgher content in basal-like breast cancer primary cells, containing CD44/CD24-/Low cells for （2. 52± 0. 58）%, but luminal a breast cancer had highest content of CD44-/CD24＋ cells,did nod contain CD44＋/CD24- /Low cells or their content was extremely low, Conclusion The stem/progenitor cells of basal like breast cancers and lu- minal-A breast cancer exhibit different phenotypes. Their differentiation ability and direction are different,which im- plied that they could be originated from different normal mammary epithelial cells.

关 键 词：乳腺癌分子亚型 乳腺癌干 祖细胞 无血清培养 细胞表型 

分 类 号：R392.12[医药卫生—免疫学]