pH值响应的共轭聚合物纳米粒子的制备、表征与细胞成像研究(英文)  

Preparation,Characterization and Cell Imaging of pH-responsive Conjugated Polymer Nanoparticles

作　　者：王建武[1] 朱春雷[1] 冯丽恒[1] 袁焕祥[1] 吕凤婷[1] 刘礼兵[1] 王树[1] 

机构地区：[1]北京分子科学国家实验室,中国科学院化学研究所有机固体重点实验室,北京100190

出　　处：《影像科学与光化学》2014年第1期121-130,共10页Imaging Science and Photochemistry

摘　　要：共轭聚合物纳米粒子(CPNs)因其高荧光亮度、低毒性、表面易修饰的特性,近年来在生物材料和生物医药领域备受关注。本论文中我们设计、合成了一种新的pH值响应共轭聚合物(PFPA),并通过纳米沉淀方法制备了其纳米粒子。动态光散射实验表明PFPA纳米粒子在水中分散性较好,其粒径约为8nm。PFPA纳米粒子的最大吸收峰为379nm,其摩尔吸光系数为2.1×106 L·mol-1·cm-1;另外该纳米粒子的荧光最大发射峰为422nm,其荧光量子产率为35%。PFPA纳米粒子在汞灯(100瓦)照射下表现出较好的光稳定性,另外MTT实验表明其具有较低的细胞毒性。该纳米粒子具有pH响应的光学特性,并可以用于活细胞成像。PFPA纳米粒子在癌症诊断、药物与基因传递等方面具有潜在的应用价值。Conjugated polymer nanoparticles （CPNs） have attracted much attention in biomaterials and biomedical fields owning to their high brightness, low cytotoxicity and easy modification properties. Herein, a new conjugated polymer PFPA was synthesized and the nanoparticles were prepared using a nano-precipitation method. Dynamic light scattering experiment indicates that PFPA nanoparticles possess small size with an average diameter of 8 nm in water. PFPA nanoparticles exhibit maximum absorption peak at 379 nm with a molar absorption coefficient of 2. 1 × 10^6 L · mol^-1 · cm^-1 and maximum emission peak at 422 nm with a fluorescence quantum yield of 35%. Moreover, PFPA nanoparticles exhibit a satisfactory photostability under the irradiation of mercury lamp （100 W）, and also display less cytotoxicity. In the aspect of applications, pH-responsive optical behavior and cell imaging ability make PFPA nanoparticles an attractive candidate for cancer diagnosis, drug and gene delivery.

关 键 词：共轭聚合物纳米粒子 荧光探针 PH响应 细胞成像 

分 类 号：TB383.1[一般工业技术—材料科学与工程]