口服避孕药拮抗雷公藤多甙致SD大鼠性腺毒性作用的研究  

The Antagonistic Effect of Oral Contracep tives on the Tripterygium Wilfordii Polyglycoside-induced Gonad Toxicity:Experimental Study of Female SD Rats

作　　者：顾江红[1] 金爱红[1] 龚智勇[1] 朱珍[1] 杜兰芳[1] 李亚琴[1] 彭皇青 

机构地区：[1]杭州市中医院妇产科,310007

出　　处：《国际生殖健康／计划生育杂志》2014年第1期11-13,F0003,共4页Journal of International Reproductive Health/Family Planning

摘　　要：目的:研究口服避孕药达英-35拮抗雷公藤多甙致SD大鼠性腺毒性作用的效果。方法:将具有正常动情周期的40只雌性SD大鼠随机分为4组:雷公藤多甙组(TWP组)、口服避孕药组(OCs组)、雷公藤多甙配伍口服避孕药组(TWP+OCs组)、空白对照组,用药10周后每日行阴道脱落细胞涂片,停药3周后处死大鼠,取腹腔动脉血用酶联免疫法测定血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成激素(LH)水平,取子宫、卵巢称湿质量,显微镜下观察子宫、卵巢的组织形态学变化及卵泡计数。结果:OCs组、TWP+OCs组与空白对照组相比较,大鼠动情周期,E2、FSH和LH水平,子宫和卵巢指数,子宫内膜和肌层厚度,卵泡和黄体计数,差异无统计学意义(P>0.05)。而TWP组大鼠大多动情周期不完整或延长,血清E2(40.417 4±4.937 2)ng/L,明显低于其余3组(P<0.01);FSH(1.063 4±0.153 9)IU/L、LH(5.946 7±1.036 0)IU/L,明显高于其余3组(P<0.01);大鼠卵巢和子宫指数分别为0.048 7%±0.009 7%和0.147 0%±0.004 7%,子宫内膜和肌层厚度分别为(57.20±1.81)μm和(24.40±1.84)μm,明显低于其余3组(P<0.051);大鼠生长卵泡(22.50±2.55)个、成熟卵泡(2.00±1.89)个,明显少于其余3组(P<0.05),但原始卵泡(36.50±2.64)个,与其余3组比较差异无统计学意义(P>0.05)。结论:配伍口服避孕药达英-35可减少雷公藤多甙对雌性SD大鼠性腺的损伤,对卵巢功能具有一定的保护作用。Objective： To study the antagonistic effect of oral contraceptives （OCs） on the tripterygium wilfordii polyglyeoside （TWP）-induced gonad toxicity in the female SD rats. Methods：40 female SD rats were divided randomly into four groups：the TWP group,the OCs group,the TWP with OCs group,and the blank control group. Test of vaginal exfoliated cell smears was done everyday after 10 weeks＇ treatment. Rats were sacrificed after stopping treatment 3 weeks. Serum follicle stimulating hormone （FSH） ,luteinizing hormone （LH）, estradiol （EL） were measured by ABC-ELISA. The weights of uterus and ovaries,and the number of follicles,were compared among groups. The histomorphology of uterus and ovary was observed by microscope. Results：The oestrous cycle in the TWP group was incomplete or extended;serum E2 level [（40.417 4±4.937 2） ng/L] was significantly lower than those in other groups;while FSH level[（1.063 4±0.153 9） IU/L] and LH levels [（5.946 7± 1.036 0） IU/L] were significantly higher than those in other groups （P〈0.01 ）. The weight indexes of uterus （0.048 7%± 0.009 7% ） and ovaries （0.147 0%±0.004 7% ）,and the thickness of endometrium [（57.20±1.81）um] and myometrium [（24.40±1.84） p.m],were significantly lower than those in other groups （P〈0.01 ）. The numbers of growing follicle （22.50±2.55） and graaffian follicle （2.00±1.89） in the TWP group were significantly less than those in the other groups （P〈0.05）,while there was not significant difference in the number of primordial follicles （36.50± 2.64） in the TWP group when compared with other groups （P〉0.05）. Conclusions：Diane-35 can decrease the TWP-induced gonad damage in female SD rats ,which plays a potential role in protecting ovarian function.

关 键 词：雷公藤属 皂苷类 避孕药 口服 性腺 达英-35 

分 类 号：R965[医药卫生—药理学]