督脉电针对脊髓损伤大鼠生长相关蛋白43表达的影响  

Effects of Du Meridian Electroacupuncture on Growth Associated Protein-43 Expression in Rats after Spinal Cord Injury

作　　者：李兵奎[1] 曾彬[2] 常巍[1] 王达义[1] 杨琪[1] 王晓勋[3] 余连军 刘涛[4] 

机构地区：[1]湖北医药学院附属太和医院脊柱外科,湖北十堰市442000 [2]湖北医药学院附属东风公司总医院内科,湖北十堰市442000 [3]湖北医药学院附属太和医院生命科学研究所,湖北十堰市442000 [4]湖北医药学院附属太和医院病理科,湖北十堰市442000

出　　处：《中国康复理论与实践》2014年第1期27-29,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：湖北省教育厅科学技术研究计划重点项目(No.D20082403)

摘　　要：目的研究督脉电针对大鼠脊髓损伤后生长相关蛋白43(GAP-43)基因及蛋白表达的影响。方法利用多中心急性脊髓损伤打击器建立Sprague-Dawley大鼠T11脊髓损伤模型,造模成功后分为对照组(A组,n=18)和督脉电针组(B组,n=18)。造模后1周,B组接受督脉电针治疗每天1次。两组大鼠于造模后1、2、4、8周行BBB后肢运动功能评分;造模后2、4、8周,采用RT-PCR检测GAP-43 mRNA表达,采用免疫组化染色检测GAP-43蛋白表达。结果造模后,与A组比较,B组BBB评分明显升高(P<0.01),GAP-43 mRNA相对表达量明显升高(P<0.01),GAP-43蛋白阳性表达明显增强(P<0.01)。结论督脉电针可促进脊髓损伤大鼠GAP-43表达。Objective To study the effect of Du meridian electroacupuncture on growth associated protein-43 （GAP-43） expression in rats after spinal cord injury （SCI）. Methods The SCI models were established with MASCIS Impactor at T11 segment in Sprague-Dawley rats. They were equally divided into control group （group A, n=18） and Du meridian electroacupuncture group （group B, n=18）. Group B re-ceived electroacupuncture once a day since 1 week after SCI. They were evaluated with the Basso-Beattie-Bresnahan （BBB） score 1, 2, 4, 8 weeks after SCI. The mRNA and protein of GAP-43 was detected with RT-PCR and immunohistochemistry 2, 4, 8 weeks after SCI. Results Compared with group A, the BBB score, the expression of GAP-43 mRNA and protein increased in group B after SCI （P〈0.01）. Conclusion Du meridian electroacupuncture can promote the expression of GAP-43 after spinal cord injury.

关 键 词：脊髓损伤 督脉电针 生长相关蛋白43 

分 类 号：R651.2[医药卫生—外科学]