孕期酒精暴露对仔鼠海马齿状回神经元增殖的影响  

Effects of alcohol exposure during pregnancy on neural cell proliferation in mouse offspring dentate gyrus

作　　者：马惠萍[1] 崔占军[2] 李峥[3] 赵凯冰[1] 

机构地区：[1]开封大学医学部解剖学教研室,开封475001 [2]河南大学医学院解剖学教研室,开封475001 [3]四川大学华西口腔医学院,成都610041

出　　处：《解剖学杂志》2013年第6期1074-1077,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（30670688,30771140）

摘　　要：目的：探讨孕期酒精暴露对子鼠（PO～P30）海马齿状回神经元细胞增殖的影响及其长时程效应。方法：从妊娠第5天（E5）开始用酒精灌胃直至仔鼠出生，建立胎儿酒精综合征动物模型。利用5-溴-2-脱氧尿苷（BrdU）并结合免疫荧光染色观察仔鼠海马齿状回神经细胞增殖的情况。结果：酒精组仔鼠海马齿状回神经元细胞增殖的数量均明显低于对照组。结论：孕期酒精暴露能够明显抑制仔鼠海马齿状回神经元细胞的增殖。Objective：To study effects of prenatal alcohol exposure on the hippocampal dentate gyrus neurons cell proliferation in mice （P0-P30）. Methods： From day fifth of pregnancy （E5） beginning with alcohol gavage until the birth of the offspring, fatal alcohol syndrome （FAS） animal ＇model was established. Using 5-bromo-2-deoxyuridine （bromodeoxyuridine, BrdU） combined with immunofluorescence to observe the mouse dentate gyrus dentate gyrus neurogenesis staining. Results： The number of mouse dentate gyrus neurons cell proliferation in the alcohol group was significantly lower than that in the control group. Conclusion. The prenatal ethanol exposure can significantly inhibit the mouse dentate gyrus of the hippocampus neuron cell proliferation.

关 键 词：胎儿酒精综合征 齿状回 神经细胞增殖 激光共聚焦显微镜 

分 类 号：R169.1[医药卫生—公共卫生与预防医学]