4种薯蓣属植物的psbA-trnH片段序列分析  

psbA-trnH Fragment Sequence Analysis of Four Species in Genus Dioscorea (Dioscoreaceae)

作　　者：郭文[1] 郭华春[1] 

机构地区：[1]云南农业大学薯类作物研究所,昆明650201

出　　处：《西北植物学报》2013年第12期2388-2394,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：云南省教育厅科学研究基金(2012J074)

摘　　要：对4种薯蓣属（Dioscorea）植物——小花盾叶薯蓣（D．sinoparviflora C．T．Ting．）、盾叶薯蓣（D．zingiberensis C．H Wright．）、黄独（D．bulbifera L．）和薯蓣（D．polystachya Turczaninow）共22个植物类群的叶绿体psbA—trnH基因区进行PCR扩增并测序，获得了该区间的完整序列，小花盾叶薯蓣的psbA—trnH片段全长589～593bp，盾叶薯蓣全长为575～599bp，黄独全长为372bp，薯蓣全长为355～368bp。将所得序列用Bayesian推断其系统发育关系，结果发现psbA—trnH片段不能够完全区分4种薯蓣属植物种间和种内的各个分类群（不同产地），且不能区分盾叶薯蓣与小花盾叶薯蓣；推测薯蓣的演变过程可能与人工驯化有关，黄独与栽培薯蓣的亲缘关系较近，而与野生薯蓣的亲缘关系较远。Four species in genus Dioscorea （D. sinoparviflora C. T. Ting. ,D. zingiberensis C. H Wright. , D. bulbifera L. ,D. polystachya Turczaninow）,22 taxa were amplified and sequenced the characteristic of psbA-trnH sequence. The length of D. sinoparviflora C. T. Ting. ,D. zingiberensis C. H Wright. ,D. bulbifera L. and D. polystachya Turczaninow fragments were 589-593 bp,575-599 bp,372 bp and 355-368 bp,respectively. According to the Bayesian phylogenetic tree, the psbA-trnH fragments couldn＇t identify the intraspecific and interspecific species successfully. D. sinoparviflora C. T. Ting. and D. zingiberensis C. H Wright. are also not distinguishable. We suppose the evolution of Dioscorea species may be related with artificial domestication. D. bulbifera L. is close to cultivated Dioscorea species.

关 键 词：薯蓣 psbA—trnH序列 序列分析 

分 类 号：Q789[生物学—分子生物学]