结瘤因子和苏芸金菌素对干旱胁迫下燕麦产量及其保护酶活性的影响  

Effects of LCO and TH17 on the Yield and Protective Enzyme Activities of Oat under Drought Stress

作　　者：胡廷会[1] 李立军[1] 李杨[1] 刘晓芳[1] 张婷婷[1] 刘景辉[1] DONALDL SMITH 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]加拿大麦吉尔大学植物科学系

出　　处：《西北植物学报》2013年第12期2451-2458,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家燕麦荞麦产业技术体系(CARS-08-B-5)

摘　　要：结瘤因子(LCO)和苏芸金菌素(TH17)在逆境环境下对作物生长具有重要调节作用。该试验在盆栽条件下,以‘坝莜3号’燕麦品种为材料,分别在苗期、拔节期和抽穗期进行干旱胁迫并叶面喷施浓度为0.01μmol/L的LCO和TH17,比较燕麦产量、叶片渗透调节物质和几种保护酶活性的变化情况。结果显示:(1)与喷施清水对照(CK)相比,正常水分条件下喷施LCO和TH17能促进燕麦生长,提高产量;在干旱胁迫下(SS)喷施LCO和TH17能减轻燕麦株高、穗长、穗粒数、生物产量、单株粒重以及干物质积累速率的降低幅度,且在苗期喷施LCO和TH17燕麦单穗粒重增加幅度最高,分别较干旱胁迫对照(SSCK)提高61.25%和65.00%。(2)干旱胁迫下,LCO和TH17使游离脯氨酸含量增加最多的时期是拔节期,分别较SSCK增加27.19%和41.00%;可溶性总糖在抽穗期提高幅度最大,分别较SSCK高61.43%和112.54%;可溶性蛋白在苗期提高幅度最大,且分别较SSCK提高9.58%和13.80%。(3)干旱胁迫下,LCO和TH17使SOD和POD活性增加幅度最大的时期均是抽穗期,CAT活性则是在拔节期增加幅度最大。(4)干旱胁迫下,在苗期LCO和TH17使燕麦叶片相对电导率和MDA含量降低幅度最大,分别较SSCK降低17.61%、36.67%和43.43%、30.28%。研究表明,在苗期喷施LCO和TH17使燕麦产量提高幅度最高;干旱条件下,不同时期喷施LCO和TH17能提高燕麦自身渗透调节能力、抗氧化保护酶活性和增强细胞膜的稳定性,从而使其更好地适应干旱条件。LCO and TH17 paly an important role under abiotic stress. In order to explore the effects of LCO and TH17 on the yield and protective enzyme activities of oat under drought stress,we conducted this experiment by foliar-spraying the oat （Bayou 3） with 0.01μmol/L concentrations of LCO and TH17 in growth cabinet with potting, to compare several changes of oat yield, osmotic regulation substances and protective enzyme activities. The results showed that. （1）The growth and development of oat were promoted under the normal water condition spraying LCO and TH17 and the yield of oats increased; under drought stress,LCO and TH17 could reduce the reduction of plant height, spike length, spike grain number, biological yield,grain weight per plant and the accumulation rate of dry materials of oats. In the seedling stage,spraying LCO and TH17 in the increasing rate of seedling stage single spike grain weight increased by 61.25% and 65.00% than that of SSCK ,respectively. （2）The LCO and TH17 could increase free proline content in elongation stage under drought stress,and increased by 27.19%and 41.00% than that of SSCK ,respectively. The highest increase rate of soluble sugar content was in the heading stage, it increased by 61.43% and 112.54% than that of SSCK ,respectively. The highest increase rate of soluble protein content was in seedling stage,and increased by 9.58% and 13.80%than that of SSCK ,respectively. （3）Under drought stress, the SOD and POD activities increased after spaying LCO and TH17 in heading stage and the CAT activity in the elongation stage. （4）Under drought stress, the LCO and TH17 could reduce the amplitude of the highest relative conductivity and MDA content in seedling stage, decreaesd by 17.61%, 36.67,43.43%,30.28% than that of SSCK,respectively. In summary,the effects of spraying LCO and TH17 in seedling stage was the largest; the LCO and TH17 can increase contents of osmotic adjustment substances and activities of protective enzymes in oat under drought stress.

关 键 词：结瘤因子 苏芸金菌素 燕麦 干旱胁迫 抗氧化系统 渗透调节物质 

分 类 号：Q945.79[生物学—植物学]