姜黄素对纳米二氧化硅诱导HaCaT细胞氧化损伤的保护作用  

Protective effects of curcumin on the oxidative damage of HaCaT cells induced by SiO_2 nanoparticles

作　　者：洪文旭 周桂凤[1] 胡韬[1] 叶金波[1] 陈晓丹[2] 张航[2] 刘建军[2] 

机构地区：[1]湖南师范大学医学院预防医学系,湖南长沙410013 [2]深圳市疾病预防控制中心深圳市现代毒理学重点实验室

出　　处：《毒理学杂志》2013年第6期412-415,共4页Journal of Toxicology

基　　金：国家自然科学基金(21102094);广东省医学科研基金资助项目(A2012577);深圳市重点实验室提升项目(ZDSY20120615085804889);深圳市科技计划项目(201102100)

摘　　要：目的研究姜黄素对纳米二氧化硅诱导HaCaT细胞损伤的保护作用。方法建立15-nm二氧化硅致HaCaT细胞损伤的体外研究模型,通过CCK-8法检测细胞活力水平;测定细胞培养液中乳酸脱氢酶(LDH)漏出量和细胞内丙二醛(MDA)含量,评价细胞的损伤程度。结果姜黄素能明显改善15-nm二氧化硅诱导HaCaT细胞的损伤,与二氧化硅组相比,姜黄素干预组能提高细胞存活率,细胞培养液中的LDH漏出量明显减少(P<0.05),细胞内的MDA明显降低(P<0.05)。结论姜黄素对15-nm二氧化硅诱导的HaCaT的细胞损伤具有一定的保护作用。Objective To study the protective effects of curcumin on the oxidative damage of HaCaT cells induced by SiO2 nanoparticles. Methods An oxidative damage model of HaCaT was induced by 15-nm SiO2. The cell viability was determined by CCK-8 method. The leakage of LDH and the level of MDA were determined to evaluate the degree of cell damage. Results The injury of HaCaT ceils induced by 15-nm SiO2 could be reduced by curcumin. Compared with the SiO2 group, curcumin could improve the survival rate and morphological change of injured cells, reduced the leakage of LDH and MDA levels. Conclusion Curcumin has significantly protective effects on the oxidative damage of HaCaT ceils induced by 15-nm SiO2.

关 键 词：姜黄素 纳米二氧化硅 HACAT细胞 细胞损伤 

分 类 号：R99[医药卫生—毒理学]