Toll样受体4基因多态性在造血干细胞移植供、受者208例中的分布研究  

The frequency of Toil-like receptor 4 polymorphisms Asp299Gly and Thr399Ile in 208 hematopoietic stem cell transplant recipients and their donors

作　　者：赵毅[1] 王丽娟[1] 罗依[1] 施继敏[1] 谭亚敏[1] 黄河[1] 蔡真[1] 

机构地区：[1]310003杭州,浙江大学医学院附属第一医院骨髓移植中心

出　　处：《中华器官移植杂志》2014年第1期13-16,共4页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金（30772502）;卫生部科学研究基金-浙江省医药卫生重大科技计划项目（WKJ2009-2-022）

摘　　要：目的探讨TLR4基因Asp299Gly和Thr399Ile多态性位点在我国部分异基因造血干细胞移植供、受者中的分布。方法以单中心2007—2012年问104对异基因造血干细胞移植供、受者为研究对象，获取了208份供者和受者的外周血样本，通过样本DNA的扩增产物直接测序法和聚合酶链反应一限制性片段多态性技术验证TLR4基因Asp299Gly和Thr399Ile位点的多态性。结果两种方法均未发现样本具有TLR4多态性位点Asp299Gly或Thr399Ile的碱基变异。结论我国部分造血干细胞移植人群中TLR4基因Asp299Gly和Thr399Ile的基因多态性非常罕见。Objective To determine the frequency of Toll-like receptor 4 （TLR4） polymorphisms Asp299Gly and Thr399Ile in a cohort of hematopoietic stem cell transplant recipients and their donors in China. Method We examined the polymorphisms in 208 peripheral blood samples collected from 104 recipients and their donors in a single center between 2007-20/2 in Zhejiang Province, China, and Asp299Gly and Thr399Ile TLR4 gene polymorphisms were detected using the sample DNA amplification products direct sequencing and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Result Both methods didn＇t demonstrate TLR4 polymorphisms Asp299Gly or Thr399Ile base mutation in our samples. Conclusion The TLR4 Asp299Gly and Thr399Ile polymorphisms are very rare in our part of the population of hematopoietic stem cell transplantation.

关 键 词：造血干细胞移植 TOLL样受体4 多态性 逆转录聚合酶链反应 

分 类 号：R457.7[医药卫生—治疗学]