丝胶对2型糖尿病大鼠海马蛋白激酶B信号转导通路的作用  

Effects of sericin on Akt signal transduction pathway of hippocampus of the rat with diabetes mellitus

作　　者：宋成军[1] 张艳[1] 和亚强[1] 陈志宏[1] 

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000

出　　处：《解剖学报》2014年第1期31-36,共6页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(H2012406018);河北省省级科技计划资助项目(2060402)

摘　　要：目的探讨丝胶(彩色蚕茧经浸泡、水煎、浓缩获得)对2型糖尿病大鼠海马蛋白激酶B(Akt)信号转导通路的作用。方法 36只雄性SD大鼠随机分为3组(n=12):正常对照组、糖尿病模型组和丝胶治疗组。小剂量链脲佐菌素(25mg/kg)连续腹腔注射(1次/d,3d)建立2型糖尿病大鼠模型。丝胶治疗组大鼠给予丝胶灌胃[2.4g/(kg·d),35d]。TUNEL法检测海马CA1区神经元凋亡,Western blotting法和RT-PCR法检测海马Akt信号转导通路相关因子Akt、核转录因子kappa B(NF-κB)和前凋亡因子Bad蛋白和mRNA的表达。结果与正常对照组比较,糖尿病模型大鼠海马CA1区神经元的凋亡指数明显升高(P<0.01);海马Akt、NF-κB的表达明显降低,Bad的表达明显升高(P<0.01)。与糖尿病模型组比较,丝胶治疗组大鼠海马CA1区神经元的凋亡指数明显降低(P<0.05);海马Akt、NF-κB的表达明显升高,Bad的表达明显降低(P<0.01,P<0.05)。结论丝胶可通过调节糖尿病模型大鼠海马Akt信号通路的异常变化减少海马神经元凋亡,对糖尿病海马损伤具有一定的拮抗作用。Objective To investigate the effects of sericin （obtained by soaking, decocting and concentrating from silkworm cocoon） on Akt signal transduction pathway of hippocampus of the rat with diabetes mellitus. Methods Thirty- six male SD rats were randomly divided into 3 groups, 12 rats per group： normal control group, diabetes mellitus model group and sericin treatment group. The type 2 diabetes mellitus rat model was established by continuous peritoneal injection of streptozotocin with a small dose （25mg/kg/d, 3d）. The rats in seriein treatment group were lavaged with sericin for 35d （2.4g/kg. d）. TUNEL was used to detect neurons apoptosis in CA1 region of hippocampus; Western blotting and RT-PCR to detect the expression of Akt signal transduction pathway related factors： Akt, NF-KB and Bad. Results Compared with normal control rats, the apoptotic index of hippocampal neurons of diabetes mellitus rats increased obviously （ P 〈 0.01 ） ; Akt and NF-KB expression in hippocampus decreased obviously, Bad expression increased obviously （ P 〈 0.01 ）. Compared with diabetes mellitus rats, the apoptotic index of hippocampal neurons of rats in sericin treatment group decreased significantly （P 〈 0. 05） ; Akt and NF-KB expression in hippocampus increased significantly, Bad expression decreased significantly （P 〈 0. O1, P 〈 0.05）. Conclusion Sericin can reduce hippoeampal neurons apoptosis by regulating abnormal changes of Akt signal transduction pathway, thus sericin has fairly protective effects on hippoeampus injury during diabetes mellitus.

关 键 词：丝胶 2型糖尿病 海马 凋亡 Akt信号转导通路 原位缺口末端标记 大鼠 

分 类 号：R587.1[医药卫生—内分泌]