苦参碱对人结肠癌HT29细胞生长抑制作用的实验研究  

The Inhibitory Effect of Matrine on the Growth of Human Colorectal Cancer HT29 Cells : an Experimental Observation

作　　者：常城[1,2] 饶德利[1] 邱晓明[1] 王弘[1] 熊莉[2] 

机构地区：[1]湖北理工学院附属黄石中心医院MRI诊疗中心,湖北435000 [2]湖北理工学院湖北省肾脏疾病发生与干预重点实验室,湖北435003

出　　处：《中国中西医结合杂志》2014年第1期62-65,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：湖北省自然科学基金资助项目(No.2010CDB07401)

摘　　要：目的研究苦参碱（matrine，MA）对人结肠癌HT29细胞系抑制和诱导凋亡的作用。方法分别采用MTT法检测细胞的生长抑制率，流式细胞仪、透射电镜观察MA处理HT29细胞后细胞周期阻滞和细胞凋亡的发生。结果2～32mg／mLMA均能抑制HT29细胞增殖（P〈0．05），其抑制率与作用时间及浓度相关。4、8、16mg／mLMA处理HT29细胞后，G。／G，细胞明显高于阴性对照组（P〈0．05），提示细胞周期停滞于G0／G1期。透射电镜可见，HT29细胞出现凋亡形态学改变。结论MA对HT29细胞有抑制作用，机制可能与阻滞细胞周期及诱导细胞凋亡有关。Objective To study the inhibitory and inductive effect of matrine （MA） on human colorectal cancer HT29 ceils. Methods MTT assay was used to determine the cell growth inhibitory rate in vitro . Changes of cell cycle and the apoptosis of HT29 cells before and after MA treatment were ob- served using flow cytometry and electron microscope. Results M-I-I- showed that 2 -32 mg/mL MA inhib- ited the proliferation of HT29 cells （P 〈0.05） in a dose-and time-dependent manner. Cells treated by 4, 8, and 16 mg/mL MA at G0/G1 phase were obviously higher than those in the negative control group （P 〈 0, 05）, indicating that the cell cycle was arrested at G0/G1 phase. Morphological apoptosis of HT29 cells could be seen under transmission electron microscope. Conclusion MA inhibited the proliferation of HT29 cells, and its mechanism might be associated with stagnation at G0/G1 phase and inducing apopto- sis of HT29 cells.

关 键 词：苦参碱 细胞凋亡 结肠癌 

分 类 号：R285.5[医药卫生—中药学]