粉防己碱对人胃癌耐药细胞锌指蛋白139、多药耐药基因表达的影响  

Effect and Mechanisms of TET on Human Gastric Carcinoma Cell Line SGC7901 and SGC7901/ADR

作　　者：李勇[1] 赵群[1] 檀碧波[1] 范立侨[1] 刘庆伟[1] 焦志凯[1] 赵雪峰[1] 郝英杰[1] 

机构地区：[1]河北医科大学第四医院外科,石家庄050011

出　　处：《中国中西医结合杂志》2014年第1期66-70,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072033);河北省自然科学基金资助项目(No.C2010000619);河北省普通高校强势特色学科资助项目(No.冀教高[2005]52);河北省卫生厅科研基金资助项目(No.20110460)

摘　　要：目的探讨粉防己碱(tetrandrine,TET)对胃癌细胞及耐药细胞中锌指蛋白139(zinc finger protein139,ZNF139)及多药耐药(multidrug resistance,MDR)基因的影响。方法 MTT法检测不同浓度(0.5、1.0、1.5、2.0、2.5μg/mL)TET对胃癌细胞SGC7901、SGC7901/ADR细胞株的抑制作用;通过RT-PCR法检测TET作用前后SGC7901/ADR细胞内ZNF139、MRP-1、MDR1、GST-π表达情况,以Spearman相关分析检验ZNF139与各多药耐药因子之间关系并计算相关系数。结果 MTT结果显示,2.0μg/mL及以下浓度TET对SGC7901、SGC7901/ADR两种细胞的抑制率均<10%;在SGC7901/ADR细胞中ZNF139、MRP-1、MDR1、GST-πmRNA表达高于SGC7901细胞(均P<0.05);TET能抑制SGC7901/ADR细胞中ZNF139、MRP-1、MDR1、GST-πmRNA的水平(均P<0.05);TET作用前SGC7901/ADR细胞中ZNF139与MRP-1、MDR1存在相关关系,作用后这种相关性仍存在(均P<0.05)。结论 TET可能通过抑制ZNF139下调、MDR1、MRP1的表达从而逆转胃癌耐药细胞MDR表型。Objective To investigate the effect of tetrandrine （TET） on zinc finger protein 139 （ZNF139） and multidrug resistance （MDR） of human gastric carcinoma cell lines and possible mecha- nisms. Methods Cultured SGC7901 and SGC7901/ADR were treated with TET （0.5, 1.0, 1.5, 2.0, and 2.5 μg/mL）, then inhibition rates were measured by M-IF assay in vitro. The expressions of ZNF139, MRP-1, MDR1, and GST-π were detected by RT-PCR. The correlation between ZNF139 and each multi- drug resistance factor was analyzed using Spearman correlation analysis, and the coefficient correlation was calculated. Results The inhibition rate of TET （ ≤2.0 μg/mL） for SGC7901 and SGC7901/ADR was less than 10% with MI-I-assay. Expressions of ZNF139, MRP-1, MDR1, and GST-μ mRNA were higher in SGC7901/ADR than in SGC7901 （all P 〈0.05）. The expressions of ZNF139, MRP-1, MDR1, and GST-πwere down-regulated in SGC7901/ADR cells efficiently （all P 〈0.01 ）. Positive correlation existed between ZNF139 and MRP-1, ZNF139 and MDR1 before treated by TET in SGC7901/ADR, and this relationship also existed in SGC7901/ADR cells after treated by TET （all P 〈0.05）. Conclusion TET could achieve MDR reversion in gastric cancer cells by down-regulating the expression of ZNF139, MRP-1, and MDRI.

关 键 词：胃癌 粉防己碱 锌指蛋白139 多药耐药 

分 类 号：R285.5[医药卫生—中药学]