人羊膜间充质干细胞对大鼠1型糖尿病模型的疗效及免疫调节作用  

Therapeutic effect and immunologic regulation of human amniotic mesenchymal stem cells in rats with experimental type 1 diabetes mellitus

作　　者：蒲莹峰 方宁[1] 陈代雄[1] 余丽梅[1] 章涛[1] 赵春华[2] 

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,563003 [2]中国医学科学院基础医学研究所组织工程中心

出　　处：《中华内分泌代谢杂志》2014年第1期55-60,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家科技重大专项课题（2009ZX09503-025）;贵州省科技计划发展项目（[2009]3017;2051）

摘　　要：目的探讨人羊膜间充质干细胞（hAMSCs）对1型糖尿病的疗效及免疫调节作用。方法分离培养hAMSCs；建立大鼠1型糖尿病模型，分为正常组、模型组、培养基组、hAMSCs组和胰岛素组，每组6只。hAMSCs组经舌下静脉注射5x10。个hAMSCs，动态监测血糖水平，检测血浆胰岛素浓度，观察胰腺病理变化和hAMSCs在胰腺中的分布，分析hAMSCs表型和淋巴细胞亚群，测定血浆Thl、Th2型细胞因子。结果与模型组比较，hAMSCs组血糖水平明显下降，血浆胰岛素含量增加，胰腺病损程度减轻：移植后6周，hAMSCs组外周血Th1和Th17细胞百分比下降（均P〈0．05），而Th2细胞和FoxP3＋Treg细胞上调（均P〈0．01）；血浆Th1型细胞因子干扰素-1、肿瘤坏死因子-α和白细胞介素（IL）-2的浓度下降（均P〈0．01），Th2型细胞因子IL4升高（P〈0．05）；hAMSCs组胰腺组织中未见由hAMSCs分化的胰岛素分泌细胞。结论hAMSCs能明显降低1型糖尿病大鼠血糖和减轻胰岛损伤，其作用机制可能主要与上调FoxP3＋Treg有关。Objective To study the therapeutic effect and immunologic regulation of human amniotic mesenchymal stem cells （ hAMSCs ） in rats with experimental type 1 diabetes mellitus （ T1 DM ）. Methods The hAMSCs from human amnion were isolated and cultured in vitro, then phenotype was analyzed by flow cytometry. T1 DM were produced by administering streptozocin to rats. The rats were divided into normal control group （ n = 6） , T1 DM model group （ n = 6 ） , medium treated group （ n = 6 ） , hAMSCs transplanted group （ n = 6 ） , and insulin treated group （n = 6 ）. 5 x 106 of hAMSCs or vehicle were administered to rats via sublingual vein. Blood glucose levels of rats were recorded weekly in the groups for six weeks by Blood Sugar Meter. At the end of 6 weeks after hAMSCs transplantation, concentrations of plasma insulin were detected by ELISA; histopathological changes of pancreas, survival and differentiation of transplanted hAMSCs in pancreatic tissue were studied with HE staining, and immunofluoreseence staining; percentages of lymphocyte subsets in peripheral blood mononuclear cells were determined by flow cytometry ; concentrations of plasma cytokines were determined by cytometric bead array. Results After hAMSCs transplantation, blood glucose levels in rats with T1DM were decreased （P 〈 0. 01 ）, while concentrations of plasma insulin were increased significantly （P〈0.01）. At 6 weeks, cell-treated animals showed an improvement in pancreas damage ; the percentages of CD4 ＋ IFN-γ＋ （ Thl ） and CIM ＋ interleukin （IL） -17 ＋ （ Thl 7 ） cells were reduced （all P〈0.05 ）, while the percentages of FoxP3-positive regulatory T cells （FoxP3 ＋Treg） and CD4＊IL-4＊ （ Th2 ） cells were increased （ all P〈0.01 ） ; plasma concentrations of interferon-γ, IL-2, and tumor necrosis factor-α were decreased （ all P〈0.01 ）, but IL-4 level was increased （ P〈0. 05 ）. No histological evidence of insulin producing cells from hAMSCs was seen within pancre

关 键 词：糖尿病 1型 人羊膜间充质干细胞 免疫调节 大鼠 

分 类 号：R587.1[医药卫生—内分泌]