精索静脉曲张对精液质量和血清、精浆中抑制素B水平的影响  

Impact of varicocele on semen quality and inhibin B concentration in serum and seminal plasma

作　　者：刘美菊[1] 王恩华[1] 

机构地区：[1]临沂市人民医院生殖医学中心,山东临沂276002

出　　处：《中华男科学杂志》2014年第1期44-47,共4页National Journal of Andrology

基　　金：临沂市科技攻关项目(201013030)~~

摘　　要：目的:探讨精索静脉曲张(VC)患者精索静脉曲张程度对精液参数和血清、精浆中抑制素B(inhibin B)水平的影响。方法:收集95例不育伴VC患者,55例有正常生育力的男性作为对照组,均通过CASA进行精液常规检查,改良巴氏染色进行精子形态检查;通过ELISA法检测外周血、精浆中抑制素B的水平。结果:VC患者正常形态精子百分率[Ⅰ度:(6.3±6.5)%,Ⅱ度:(2.6±3.0)%,Ⅲ度:(1.0±0.7)%]和前向运动精子百分率[Ⅰ度:(33.3±20.8)%,Ⅱ度:(28.9±19.8)%,Ⅲ度:(13.5±8.4)%]明显低于对照组[(7.5±5.2)%,(43.9±22.7)%](P<0.05),精子畸形以头部畸形为主;VC患者外周血和精浆中抑制素B水平[Ⅰ度:(160.9±48.9)pg/ml,(208.3±28.1)pg/ml;Ⅱ度:(150.6±44.7)pg/ml,(201.5±83.5)pg/ml;Ⅲ度:(132.6±41.5)pg/ml,(150.2±51.6)pg/ml]均低于对照组[(201.0±38.1)pg/ml,(225.3±82.5)pg/ml]。结论:VC可导致精子活力降低,畸形率升高,外周血和精浆抑制素B水平下降,从而导致男性生育力受损。Objective ： To investigate the influence of varicocele （VC） on semen parameters and the concentration of inhibin B in the serum and seminal plasma of VC men. Methods ： We collected semen and peripheral blood samples from 95 infertile VC patients and 55 normal fertile men. We performed semen routine examination by computer-assisted semen analysis and sperm morphology examination by modified Papanicolaou staining, and measured the levels of inhibin B in the peripheral blood and seminal plasma of the subjects by ELISA. Results： Compared with the normal fertile controls, the infertile men with grade- I , - II and -III vc showed significantly lower percentages of morphologically normal sperm （ [ 7.5 ± 5.2 ] % vs [ 6.3 ± 6.5 ] %, [ 2.6 ± 3.0 ] % and [ 1.0 ± 0.71%,P〈0.05） and progressively motile sperm （[43.9 ±22.7]% vs [33.3 ±20.81%, [28.9 ±19.81% and [13.5 ± 8.4 ] %, P 〈0. 05 ）. The majority of the morphologically abnormal sperm were of the type of head deformity. The concentrations of inhibin B in the peripheral blood and seminal plasma were evidently lower in the infertile men with grade- I VC （ [ 160.9 ＋ 48.9 ] pg/ml and [208.3 ±28.1] pg/ml）, grade-l/ VC （[150.6±44.7] pg/ml and [201.5±83.5] pg/ml）, and grade-III VC （[132.6± 41.5 ] pg/ml and [ 150.2 ± 51.6 ] pg/ml） in comparison with those of the fertile control group （ [ 201.0 ± 38.1 ] pg/ml and [ 225.3 ± 82.5 ] pg/ml）. Conclusion： Varicocele reduces sperm motility, increases sperm abnormality, decreases the concentration of inhibin B in the serum and seminal plasma, and consequently damages male fertility.

关 键 词：抑制素B 精索静脉曲张 精液参数 

分 类 号：R698.2[医药卫生—泌尿科学]