成都地区儿童腹泻症候群人博卡病毒监测及基因变异分析  

Detection and analysis of genetic diversity of human bocavirus in diarrhea children in Chengdu

作　　者：周涛[1] 杨晶艳[2] 杜晓昕[1] 陈凡[1] 董泽丰 刘娱[1] 许欣[1] 裴晓方[1] 

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]成都中医药大学医学技术学院微生物与免疫学检验教研室,四川成都611137

出　　处：《现代预防医学》2014年第3期520-522,524,共4页Modern Preventive Medicine

基　　金：“艾滋病和病毒性肝炎等重大传染病防治科技”重大专项“传染病检测技术平台”项目;“云南及周边省市区传染病病原谱流行规律研究”课题(2012ZX10004-212)

摘　　要：目的了解成都市婴幼儿腹泻患者人博卡病毒检出率、亚型分布以及VP1基因变异特征。方法收集四川大学华西第二医院2010年4月-2012年9月期间儿童腹泻感染病例标本共计749例,运用PCR方法检测HBoV VP1 DNA,随机选取25例阳性标本进行VP1基因测序,构建进化树并比较其与基因文库参考序列之间的差异。结果 HBoV的检出率为25.77%(193/749),随机选取的25例阳性标本中,检出HBoV2和HBoV3各1例,其余23例为HBoV1。各亚型阳性标本VP1基因核苷酸序列与参考序列相比均没有明显差异,其中HBoV1亚型VP1基因与其参考序列相似性大于99%,HBoV2为97.5%,HBoV3为98.6%。结论成都地区婴幼儿腹泻患者博卡病毒检出率较高,以HBoV1亚型为主。Objective To investigate the infection rate, genotype and VP1 gene mutation characteristics of human bocavirus （HBoV） infection in children with diarrhea in Chengdu. Methods Fecal samples were collected from 749 children with diarrhea from April, 2010 to September, 2012 at West China Second University Hospital, Sichuan University. The HBo~ ~P1 DNA from all the samples was detected by PCR. The VP1 genes amplified from randomly selected positive samples were sequenced and compared with the reference sequence found in GenBank by constructing the phylogeny tree. Results Out of 749 fecal samples, 25.77% （193/749） were positive for HBoV. After phylogenetic analysis, the result demonstrated that 23 samples were HBoV1 positive, but only one HBoV2 and HBoV3 positive were observed in 25 randomly selected positive samples. No obvious differences were found between the reference sequence and the amplified ones. The similarity of HBoV1, HBoV2 and HBoV3 with each reference sequence was 99%, 97.5% and 98.6%. Conclusion In Chengdu region, HBoV is highly detected in diarrhea children. HBoV1 is the main subtype.

关 键 词：婴幼儿腹泻 人博卡病毒 VP1基因 

分 类 号：R722.132[医药卫生—儿科]