硫化氢对糖尿病大鼠ED的影响和机制研究  

Role of hydrogen sulfide in erectile dysfunction in diabetic rats

作　　者：毛玉山[1] 叶小磊 麦一峰[3] 王少敏[4] 洪中立[3] 王黎明[1] 赵廷启[1] 王铁樵 吕迪[1] 何文明[3] 

机构地区：[1]宁波大学医学院附属医院内分泌科 [2]宁波市医学科学研究所药物与药理研究室 [3]宁波大学医学院附属医院心内科 [4]宁波大学医学院附属医院肿瘤内科,浙江宁波315020

出　　处：《中国病理生理杂志》2014年第1期139-143,共5页Chinese Journal of Pathophysiology

基　　金：宁波市自然科学基金资助项目(No.2012A610187);宁波市社会发展计划项目(No.2011C50021;No.2013C50044);宁波市"宁大附属医院糖尿病防治特派员团队"项目(No.2013C80103)

摘　　要：目的:观察硫化氢(H2S)对糖尿病(DM)大鼠勃起功能障碍(ED)的影响。方法:将70只SD雄性大鼠分为2组,60只腹腔注射链脲佐菌素(STZ)建立DM大鼠模型,10只注射生理盐水为正常对照。阿朴吗啡诱导勃起后检测DM造模成功大鼠的勃起功能情况,将存在ED的36只大鼠随机分成3组:糖尿病对照组(DM组)、糖尿病+硫氢化钠组(DM+NaHS组)和糖尿病+西地那非组(DM+sildenafil组)。DM+NaHS组每天腹腔注射NaHS(50μmol/kg),DM+sildenafil组每天给予西地那非(5 mg/kg)灌胃,DM组每日腹腔注射生理盐水,注射体积为0.25 mL。持续饲养12周后,观察各组大鼠勃起功能情况,采集各组大鼠动脉血,测定血液中H2S含量及各组阴茎海绵体组织胱硫醚γ-裂解酶(cystathionineγ-lyase,CSE)活性及CSE表达水平。结果:STZ诱发的DM造模成功率为81.7%(49/60),DM大鼠发生ED的比例为73.5%(36/49),与DM组比,DM+NaHS组和DM+sidenafil组大鼠勃起功能良好,3个实验组大鼠勃起率分别为(33.3±5.5)%、(54.5±5.3)%和(63.6±9.1)%。与正常大鼠组比,各组DM大鼠阴茎海绵体中CSE活性与表达水平均有下降,其中DM+NaHS组大鼠的CSE活性及表达水平显著低于DM组(P<0.05)和DM+sildenafil组(P<0.05)。结论:STZ诱导的糖尿病大鼠中ED发生率较高,H2S对糖尿病合并ED大鼠勃起功能有一定的促进作用。外源性给予H2S可以反馈性抑制大鼠阴茎组织中CSE的活性和表达。AIM： To study the effects of hydrogen sulfide （H~ S） on erectile dysfunction （ED） in a diabetic rat model. METHODS ： Diabetes mellitus （DM） was induced in 60 male adult Sprague-Dawley rats by intraperitoneal in- jection of streptozocin （STZ） and 49 DM rats were established, in which 36 DM rats were accompanied with ED by detec- ting apomorphine-induced erections. The DM ＋ ED rats were further randomly divided into 3 groups： DM group, DM ＋ NariS group and DM ＋ sildenafil group. Meanwhile, another 10 normal SD rats were used as control group. The rats in DM ＋ Naris group were treated with Naris at dose of 50 i^mol/kg by intraperitoneal injection everyday for 12 weeks. The rats in DM ＋ sildenafil group were gavaged with sildenafil at dose of 5 mg/kg every day for 12 weeks. Equal volume of ster- ile saline was injected in the rats in normal control group and DM group. The erectile function of all rats was evaluated after injection of apemorphine. The blood concentration of H2S was detected. Furthermore, the activity and expression level of cystathionine ,/-lyase （CSE） in the penile tissue were investigated. RESULTS： STZ-induced DM had a successful rate of 81.7% （49/60）, and the proportion of DM rats with ED was 73.5% （36/49）. Compared with DM group, the rats in DM ＋ Naris group and DM ＋ sildenafil group had a better erectile function, with erection frequencies of （33.3 ＋ 5.5 ）%, （54.5 ＋ 5.3） % and （63.6 ~ 9.1 ） %, respectively. Compared with the normal rats, the activity and expression level ofCSE in all DM groups were decreased, which were also significantly lower in DM ＋ NariS group than those in both DM group （ P 〈 0.05 ） and DM ＋ sildenafil group （ P 〈 0.05 ）. CONCLUSION： STZ-induced diabetic rats have a high inci- dence of ED. H2S plays a certain role in promoting erectile function in diabetic rats with ED. Exogenous administration of H2S may establish a negative feedback inhibition on the activity and expression of CSE in rat p

关 键 词：硫化氢 糖尿病 勃起功能障碍 胱硫醚γ-裂解酶 

分 类 号：R587.1[医药卫生—内分泌]