肺炎球菌溶血素溶血活性致大鼠脑损伤机制研究  

Mechanism of hemolytic activity in pneumolysin-induced brain injury in rats

作　　者：禚志红[1] 刘敏[1] 王怀立[1] 

机构地区：[1]郑州大学第一附属医院儿科

出　　处：《医学研究生学报》2014年第1期30-33,共4页Journal of Medical Postgraduates

摘　　要：目的溶血活性是肺炎球菌溶血素(pneumolysin,PLY)的重要生物学特性之一。运用不同溶血活性的PLY建立脑损伤模型,探讨溶血活性对PLY致大鼠脑损伤的作用机制。方法 4周龄大鼠,经左侧颈内动脉注入等剂量溶血活性缺失的PLY[PLY(-)]及溶血活性正常的PLY[PLY(+)],观察脑组织神经损伤情况及炎症因子水平。结果 PLY(+)组脑组织神经元烯醇化酶(Neuron-specific enolase,NSE)[(122.495±4.852)%]及胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)水平[(135.697±2.727)%]较PLY(-)组及等渗盐水(NS)对照组[(80.467±2.460)%,(89.622±3.226)%]均明显升高,差异有统计学意义(P<0.05),PLY(-)组NSE[光度值(102.081±2.150)%]及GFAP水平[光度值(96.323±4.113)%]也高于对照组(NSE光度值80.467±2.460,GFAP光度值89.622±3.226),差异有统计学意义(P<0.05);PLY(+)组TNF-α及IL-6水平[(103.902±3.221)、(52.030±1.817)ng/mL]较PLY(-)组[(94.152±3.032)、(41.628±2.972)ng/mL]及NS对照组[(69.986±3.567)、(21.246±3.118)ng/mL]均明显升高,差异有统计学意义(P<0.05),PLY(-)组TNF-α[(94.152±3.032)ng/mL]及IL-6水平[(41.628±2.972)ng/mL]也较NS对照组[(69.986±3.567)ng/mL,(21.246±3.118)ng/mL]明显升高,差异有统计学意义(P<0.05)。结论溶血活性在肺炎链球菌造成脑损伤过程中具有重要作用,并且一定程度上可促进炎性因子释放。Objective Hemolytic activity is one of the important biological characteristics of pneumolysin （PLY）. This study was to explore the mechanism of hemolytic activity in rat models of brain injury induced by PLY with different hemolytic activi- ties. Methods Thirty 4-week-old rats were divided into 3 groups of equal number to be injected via the left internal carotid artery with isodose PLY without hemolytic activity PLY（ - ）, PLY with normal hemolytic activity, PLY（ ＋ ） and normal saline （ NS con- trol）, respectively, followed by observation of nerve injury and the level of cytokines in the brain tissue. Results Compared with the PLY （ - ） （ [ 80. 467± 2.460 ] % ） and NS control groups （ [ 89. 622 ± 3. 226 ）, the PLY （ ＋ ） （ [ 135. 697± 2. 727 ] % ） group showed significantly increased levels of neuron-specific enolase （NSE） and glial fibrillary acidic protein （GFAP） in the brain tissue （ P 〈 0.05 ）, and higher in the PLY （ - ） group than in the control （ P 〈 0.05 ）. The levels of TNF-aand IL-6 were also significantly increased in the PLY （ ＋ ） （ [ 103. 902±3. 221 ], [ 52. 030 ± 1. 817 ] ng/mL） group in comparison with the other two （ P 〈 0.05 ）, and higher in the PLY（ - ） group than in the control （P 〈 0.05 ）. Conclusion Hemolytic activity plays an important role in pneumo- coccus-induced brain injury and, to a certain extent, promotes the release of cytokines.

关 键 词：肺炎球菌溶血素 脑损伤 溶血活性 

分 类 号：R651.15[医药卫生—外科学]