水稻MYB转录因子冷胁迫表达谱分析  

Expression Profiling of MYB Transcription Factors in Rice under Chilling Stress

作　　者：刘晓月[1] 张婷[1,2] 黄立钰[1] 王文生[1] 傅彬英[1] 

机构地区：[1]中国农业科学院作物科学研究所,农作物基因资源和遗传改良国家重大科学工程,北京100081 [2]杂交水稻国家重点实验室,武汉大学生命科学院,武汉430072

出　　处：《分子植物育种》2014年第1期9-16,共8页Molecular Plant Breeding

基　　金：国家转基因专项项目(2011ZX08001-003)资助

摘　　要：本论文以耐冷粳稻品种丽江新团黑谷及冷敏感籼稻品种IR29为研究材料,在4℃低温条件下进行处理,采用荧光定量PCR技术分析MYB转录因子候选基因的表达模式。研究发现低温胁迫条件下,45个MYB基因在两个品种中的表达谱存在较大差异,鉴定了部分耐冷品种特异冷胁迫差异表达MYB基因;另外有小部分MYB基因在两个品种表达方式一致,但表达量存在较大差异。结果表明MYB基因低温胁迫下的不同表达方式或差异表达量可能与两个品种的不同耐冷性相关,研究为进一步分析耐冷候选MYB基因功能打下了基础。In present study, the quantitative real-time PCR was used to profile the MYB transcription factors under chilling stress in two rice genotypes of LTH and IR29 with contrasting chilling tolerance. Results showed that most of the MYB genes were differentially expressed under low temperature condition, and a set of MYB genes were specifically regulated in chilling tolerant LTH. A portion of MYB genes have the same change tendency in both varieties with diverse expression level under stress. All these results showed that differentially regulated MYB genes might be related to the genotype-specific chilling stress tolerance, and the data will provide a basis for further functional dissection of chilling tolerance MYB genes in rice.

关 键 词：水稻 低温胁迫 MYB转录因子 定量PCR 

分 类 号：S511[农业科学—作物学]