大豆百粒重相关分子标记的实用性分析与验证  

Practical Analysis & Verification of Molecular Marker of Weight of 100-seed in Soybean

作　　者：任海红[1] 刘学义[1] 朱保葛[2] 林汉明[3] 

机构地区：[1]山西省农业科学院经济作物研究所,汾阳032200 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]香港中文大学生命科学学院,香港999077

出　　处：《分子植物育种》2014年第1期69-73,共5页Molecular Plant Breeding

基　　金：国家大豆产业技术体系和山西省百人计划项目共同资助

摘　　要：本研究从全国各地收集到不同生态类型大粒材料36份,小粒材料12份。运用已经定位的分子标记共7对,经PCR扩增,用9%丙烯酰胺凝胶电泳,统计每个SSR位点,最后采用SPSS软件作方差分析。结果显示:选出3个与大粒相关的实用性标记,分别为GNE070、Satt126和Satt633。可以被一个或一个以上标记检出的大粒材料占35(大粒材料共36份),检出率高达97.2%。研究结果表明用GNE070、Satt126和Satt633联合检测,可以应用于分子标记辅助育种大粒育种实践。48 soybean （36 large grain varieties and 12 small grain varieties） varieties are collected from many areas of China, including different ecological types. 7 pairs of location-known molecular markers are used for syste- matically practical confirmation and value evaluation. Amplified by PCR, ran electrophoresis with a 9% acryla- mide gel, counted each SSR loci and finally analysised variance with SPSS software. The results are as follows： 3 practical molecular markers （GNE070, Satt126, Satt633） were selected in the large/small grain group. In short, 35 materials can be detected by one or more markers （the number of large-seed material is 36）, and the coincident rate is as high as 97.2%. The experimental results show that the joint use ofGNE070, Satt126 and Satt633 is feasible in molecular marker-assisted breeding （large-grain breeding） practice.

关 键 词：大豆 百粒重 分子标记 实用性 

分 类 号：S565.1[农业科学—作物学]