生长分化因子-9(GDF9)基因多态性与邵伯鸡母系产蛋性状的关联性分析  

Associations of Growth Differentiation Factor 9 Gene's Polymorphisms with Egg Production Traits in Female Line of Shaobo Chicken(Gallus domesticus)

作　　者：李春苗[1] 黎寿丰[1] 黄华云[1] 赵振华[1] 王钱保[1] 薛龙岗[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225125

出　　处：《农业生物技术学报》2014年第1期71-78,共8页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系建设专项No.CARS-42-Z06);江苏省科技支撑项目(No.BE2013386);江苏省高校重点实验室开放课题(No.K12039)

摘　　要：为探讨生长分化因子-9基因(GDF9)的多态性与邵伯鸡母系产蛋性状的关系,本研究以中国农业科学院家禽研究所选育的216只邵伯鸡(Gallus domesticus)母系为材料,利用PCR-RFLP技术检测GDF9基因的SNPs,并分析基因多态性对邵伯鸡母系开产体重、开产日龄、开产蛋重、300日龄总产蛋数以及300日龄平均蛋重之间的关系。结果显示,在外显子2发现3个突变位点,G2051A位点检测到3种基因型GG、GA和AA,相关分析显示,GG基因型个体的开产体重和开产蛋重显著低于AA型个体(P<0.05),而300日龄产蛋数显著高于AA型个体(P<0.05);T2273C位点检测到2种基因型TT和TC,TT基因型个体与CT基因型个体的各性状差异均不显著(P>0.05),C2336T位点检测到3种基因型CC、CT和TT,CT基因型个体的开产日龄显著早于CC与TT型个体(P<0.05),其300日龄产蛋数显著高于CC型与TT型个体(P<0.05)。3个位点的联合基因型GGCTCT对开产体重、开产日龄、开产蛋重、300日龄平均蛋重、300日龄产蛋数均有显著影响。研究结果提示,邵伯鸡母系GDF9基因的多态性与产蛋性状呈显著相关,可尝试用联合基因型GGCTCT为筛选邵伯鸡母系产蛋性状的分子标记辅助选择。This experiment was designed to study the effects of polymorphism of growth differentiation factor 9 gene （GDF9） on egg production traits in female line of Shaobo chicken（Callus domesticus）, and 216 female line of Shaobo chicken breeding in Poultry Institute, Chinese Academy of Agricultural Sciences were used in the present study. The genetic polymorphism of GDF9 was investigated by PCR-RELP, and analyze the association of polymorphisms sites with egg production traits in female line of Shaobo chicken, which included age at first egg （AFE）, body weight at first egg, weight at first egg （WFE）, average egg weight at 300 days （AWEN） and the total number of eggs at the age of 300 days （EN）. The results indicated that three SNPs site were detected in exon2 of GDF9 gene. Three genotypes（AA, AG and GG） were found at G2051A mutation locus, two genotypes（CT, TT） were found at T2273C mutation locus, three genotype（CC, CT,TT）were found at C2336T mutation locus. The fitness test showed that all Shaobo chicken at three mutation locus were in Hardy-Weinberg equilibrium. The relationship between GDF9 gene and reproduction traits in female line of Shaobo chicken was analyzed by least squares linear model. The individuals with GG genotype had significant lighter weight at first egg （P〈0.05）, GG genotype birds had also significant more the total number of eggs at the age of 300 d （P〈0.05）. Least squares means of reproduction traits for TT was not significantly lower than that for CT （P〉0.05） at T2273C mutation locus. CT genotype birds had significant younger age at first egg （P〈0.05）, the individuals with CT genotype had also significant more the total number of eggs at the age of 300 d （P〉0.05）. The combine genotypes also had significant genetic effects on age at first egg （AFE）, weight at first egg （WFE）, body weight at first egg （BWFE） and the total number of eggs with 300 days （EN）. However, it seemed that the effect of GDF9 gene with combine gen

关 键 词：邵伯鸡母系 长分化因子-9(GDF9) 产蛋性状 

分 类 号：S813.3[农业科学—畜牧学]