青海省2011-2012年乙型流感病毒HA1基因特性研究  

Molecular characteristic of haemagglutination gene of influenza B virus in Qinghai Province,2011-2012

作　　者：于娟[1] 饶华祥[1] 李红[1] 卢囡囡 易虎[1] 赵生仓 

机构地区：[1]青海省疾病预防控制中心,西宁810007

出　　处：《中国人兽共患病学报》2014年第1期32-35,共4页Chinese Journal of Zoonoses

基　　金：国家"十一五"科技重大专项(2009ZX10004-208)~~

摘　　要：目的了解2011—2012年青海省乙型流感病毒HA1基因变异情况。方法随机选择2011—2012年流感病原监测中分离到的乙型流感毒株，提取病毒RNA，通过RT—PCR法扩增病毒HA1基因，纯化产物进行核苷酸序列测定，采用DNA Star5．0MegAlign和MEGA4．0生物软件对测序结果进行分析处理，推导出氨基酸序列并进行基因特性分析。结果12株Victoria系乙流感病毒HA1区域核苷酸序列与2009—2011年WHO推荐疫苗株B／Brisbane／60／2008同源性为88．6％～95．7％，氨基酸替换数在2～5个，所有分离株均在1个相同的氨基酸位点发生了相同的替换，部分分离株在320位减少了一个糖基化位点。5株Yamagata系乙型流感病毒HAl区域核苷酸序列与2011-2012年WHO推荐疫苗株B／Wisconsin／01／2010HA1区相比较同源性为97．7％～99．3％，氨基酸替换数在2—3个，所有分离株均在2个相同的氨基酸位点发生了相同的替换（N116K，D196N），在196位增加了一个糖基化位点。结论青海省2011—2012年乙型流感病毒HA1基因特性正在逐渐发生变异，但尚未形成抗原漂移，今后在流感监测工作中要加强流感病原学的监测，及时发现新的乙型流感病毒变异株。In order to understand the genetic characterizations of haemagglutination of influenza B viruses isolated from 2011 to 2012 in Qinghai Province, we selected Influenza B virus strains isolated randomly during 2011 to 2012, then the RNAs were extracted and HA1 genes were amplified by reverse transcription polymerase chain reaction （RT-PCR）. The purified PCR products were sequenced by genetic analyzer and analyzed by DNAStar 5.0 MegAlign and MEGA 4.0. It was shown that HA1 gene of 12 strains of Yamagata-like viruses had identity of 88.6-95.7% in nucleotide sequence compared with vaccine strain rec- ommended by WHO （B/Brisbane/60/2008） during 2009- 2011, and had 2-5 amino acid substitutions, in which one amino acid substitution occurred in all isolates and a glycosylation site missed at site 320 in some isolates. Moreover, HA1 gene of 5 strains of Yamagata-like viruses had identity of 97.7-99.3 % in nucleotide sequence compared with vaccine strain recommended by WHO （B/Wisconsin/01/2010） during 2011-2012, and had 2-3 amino acid substitutions, in which 2 amino acid substitutions occurred in all isolates and a glycosylation site added at site 196 in some isolates. In conclusion, HA1 gene of influenza B virus isolated during 2011-2012 has being changed gradually, but no antigenic drift formed. It＇s suggested that we should strengthen the etiology surveillance of influenza virus, which would promote new influenza B virus variants be found as soon as possible.

关 键 词：乙型流感病毒 HA1基因 抗原决定簇 糖基化位点 

分 类 号：R373.9[医药卫生—病原生物学]