星点设计-效应面法优选湖北海棠叶总黄酮的提取工艺  

Optimization of Extraction Technology for Total Flavone of Malus Hupehensis Leaves by Central Composite Design /response Surface Methodology

作　　者：郭东艳[1] 王幸[1] 唐志书[1] 汪鋆植 

机构地区：[1]陕西中医学院药学院,咸阳712046 [2]天然产物研究与应用湖北省重点实验室,宜昌443002

出　　处：《医学研究杂志》2014年第1期23-27,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81202905);陕西省教育厅基金资助项目(11JS036)

摘　　要：目的优选湖北海棠叶总黄酮的提取工艺。方法采用星点设计-效应面法对湖北海棠叶有效部位的提取工艺进行优化,以乙醇浓度、溶剂用量、提取时间为自变量,以总黄酮和根皮苷含量的综合评分为因变量,将各数据输入软件中,从而从图上的较佳区域直接读出最佳的工艺条件。结果湖北海棠叶总黄酮的提取工艺条件为:用8倍量70%乙醇,回流提取2次,每次150min。结论优选的工艺参数合理、可行。Objective To optimize the extraction process of total flavones of Malus hupehensis leaves. Methods The central com- posite design/response surface methodology was used. The independent variables were ethanol concentration, solvent usage, and reflus time, and the dependent variables were the content of total flavones and phlorizin. Data were input into the software. The optimum extraction conditions was fetched directly from the preferable area of the chart. Results The optimum conditions of extraction process were 70% eth- anol,reflux for 150 minutes,8 - fold solvent and 2 times for extraction. Conclusion This optimized extraction process parameter was con- venient and feasible.

关 键 词：湖北海棠叶 总黄酮 提取 

分 类 号：R284[医药卫生—中药学]