烫伤大鼠小肠黏膜组织HSP70的表达与细胞凋亡的变化规律研究及山莨菪碱的影响  

Effect of Anisodamine on Expression of HSP70 and the Apoptosis in Small Intestinal Epithelium Cells of Scalded Rats During Early Postburn Stage

作　　者：刘政军[1] 葛茂星[2] 付晋凤[2] 刘文军[2] 卢才教[1] 林才[1] 郭海雷[1] 

机构地区：[1]温州医科大学附属第一医院烧伤科,325000 [2]昆明医学院附属第二医院烧伤科

出　　处：《医学研究杂志》2014年第1期30-34,共5页Journal of Medical Research

基　　金：云南省卫生科技计划基金资助项目(2009NS084)

摘　　要：目的研究烫伤后早期给山莨菪碱(654-2)对大鼠小肠黏膜组织细胞热休克蛋白70(heat shock protein,HSP70)表达及细胞凋亡的影响。方法采用随机数字表法将SD大鼠分为单纯烫伤组(烧伤组)、烫伤后654-2治疗组(治疗组),SD大鼠30%TBSA 3度烫伤,伤后不同时相点,取小肠黏膜组织。以免疫组织化学的方法检测分析各组小肠组织黏膜HSP70的表达情况,以TUNEL法检测分析各组小肠组织黏膜细胞凋亡情况,同时观测各组小肠组织黏膜的病理形态学变化。结果伤后大鼠小肠黏膜组织细胞凋亡增加,治疗组凋亡指数下降,同时细胞内HSP70表达增加。结论严重烧伤早期SD大鼠小肠黏膜组织损伤明显,小肠黏膜细胞凋亡可能是严重烧伤早期大鼠小肠黏膜组织损伤的重要原因之一,早期应用654-2对烧伤早期SD大鼠小肠黏膜组织损伤具有明显保护作用。山莨菪碱对小肠黏膜组织的保护作用可能与其增强细胞内HSP70表达、抗小肠黏膜细胞凋亡有关。Objective To observe the pathological changes,the expression of Heat Shock Protein（HSP70） and the apoptosis in small intestinal epithelium after burn injury and the treatment of anisodamine. Methods The scalded rats were randownly divided into 2 groups：simple burn group（ group A）, anisodamine ＋ burn group （group B）. The intestinal tissue was determined at 3,6,12,24 and 48 hour after 50% TBSA Ⅲ degree scald. HSP70 expression in small intestinal mucosa was determined by immunihistochemistry. The patho- logical changes of intestinal tissues were observed by HE. Results Apoptotic index of group A was increased after scald. Apoptotic index of group B were relieved obviously,And the expression of HSP70 of group B was increased at the same time. Conclusion The small intes- tinal epithelium tissues of SD rats were damaged obviously during the early burning stage,and maybe the apoptosis in small intestinal epitheli- um ceils is one of the most important reasons in the damage of small intestinal epithelium tissues of SD rats after burn injury. Anisodamine can prevent the apoptosis in small intestinal epithelium cells and relieve the damage of small intestinal epithelium tissues in the early stage of burning. Anisodamine can enhance the expression of HSP70 in small intestinal epithelium tissues of scalded rats. Overexpression of HSP70 in- duced by anisodamine maybe an important mechanism for anisodamine to prevent the apoptosis and protect intestinal mucus barrier.

关 键 词：山莨菪碱(654—2) 热休克蛋白70 凋亡 烧伤 肠黏膜屏障 

分 类 号：R644[医药卫生—外科学]