LPS对THP-1细胞sCD14和mCD14表达的影响  

Effect of LPS on THP-1 Expressing sCD14 and mCD14

作　　者：张颖[1] 王金文[2] 李向阳[3] 

机构地区：[1]温州市中西医结合医院,325000 [2]大庆油田总医院,163000 [3]温州医科大学附属第二医院,325000

出　　处：《医学研究杂志》2014年第1期41-44,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y20080596);温州市科技计划项目(Y20100094)

摘　　要：目的观察不同浓度脂多糖(LPS)刺激THP-1细胞(human acute monocytic leukemia cell line)不同时间对可溶性CD14(soluble CD14,sCD14)和膜CD14(Membrane CD14,mCD14)表达的影响及sCD14、mCD14表达之间的关系。方法不同浓度LPS(0、0.1、1.0、10、50ng/ml)刺激经佛波酯(PMA)诱导的THP-1细胞不同时间(0.5、1、3、5、8h),用酶联免疫吸附法检测sCD14的量,流式细胞术检测mCD14的量。结果 LPS浓度为0ng/ml时,sCD14的表达与时间呈正相关(r=0.915,P=0.029),8h达最高值(1044.39±12.21pg/ml),mCD14的表达与时间呈正相关(r=0.945,P=0.015),8h达最高值(4.23%±2.11%),且随着时间的延长,sCD14的表达与mCD14的表达呈正相关(r=0.969,P=0.006);LPS浓度为0.1ng/ml时,随着时间的延长,sCD14的表达与mCD14的表达呈正相关(r=0.944,P=0.016);当LPS刺激细胞0.5h时,sCD14的表达与mCD14的表达呈负相关(r=-0.839,P=0.037);1h时,mCD14的表达与LPS浓度呈正相关(r=0.839,P=0.037),100ng/ml时达最高值(3.24%±1.95%)。结论未经LPS刺激的THP-1细胞可以表达sCD14及mCD14;LPS的浓度及刺激细胞的时间促进sCD14和mCD14表达。Objective To investigate the effect of different concentrations of LPS on THP - 1 cell expressing soluble CD14 and mCD14 for different time. Methods Cultured THP - 1 monocyte cell was induced by PMA to macrophage and stimulated it with different concentrations of LPS（0,0.1,1.0,10 and 50ng/ml） for different time（0,0.5,1,3,5 and 8h）. Then sCD14 was detected by Enzyme- linked immunosorbent assay,and mCD14 by Flow cytometry. Results When the concentration of LPS was 0ng/ml,sCD14 enhanced along time（ r = 0. 915 ,P = 0. 029） , and reached the highest value（ 1044.39 ± 12.21pg/ml） at 8h. When LPS concentration was 0ng/ml, mCD14 enhanced as time extended（ r = 0. 945, P = 0. 015 ） , and reached the highest value （4.23 % ± 2.11% ） at 8h. And as time extended, the expression of sCD14 and mCD14 were positively correlated（r = 0. 969, P = 0. 006）. When LPS concentration was 0.1 ng/ml, sCD14 and mCD14 had a positive correlation as time extended（ r = 0. 944,P = 0. 016）. When LPS stimulated ceils 0.5h, sCD14 and mCD14 had a negative correlation with LPS concentration increasing（ r = - 0. 839, P = 0. 037 ）. At lh, mCD14 enhanced as the LPS concentration in- creasing（r =0. 839, P = 0. 037）. And at 100ng/ml it reached the highest value （3.24% ± 1.95% ）. Conclusion THP- 1 cells in- duced by PMA spontaneously expressed sCD14 and mCD14 without LPS stimulation. The concentration of LPS and stimulation time in- creased the expression of sCD14 and mCD14.

关 键 词：LPS THP-1细胞 SCD14 MCD14 

分 类 号：R392[医药卫生—免疫学]