沙棘黄酮对动脉粥样硬化大鼠血管的保护作用及机制  

Protective Effect and Mechanism of TFH Against Damages in Atherosclerosis Rats.

作　　者：王颖超[1] 柳茵[2] 刘维军[2] 李琳[2] 

机构地区：[1]青海大学医学院,西宁810001 [2]青海大学附属医院

出　　处：《医学研究杂志》2014年第1期90-93,共4页Journal of Medical Research

摘　　要：目的研究沙棘黄酮(TFH)预防高脂饮食所致大鼠动脉粥样硬化的分子机制。方法按随机数字表法将50只雄性SD大鼠分为空白对照组、粥样硬化组、沙棘黄酮低、中、高剂量组,喂养2个月后,提取血清分析谷胱甘肽过氧化物酶(GSHPx)、过氧化氢酶(CAT)活性和超氧化物歧化酶(SOD)、丙二醛(MDA)含量;取主动脉后,用HE染色法观察血管损伤程度,采用荧光试剂盒测定血管活性氧簇(ROS)水平,ELISA技术测主动脉斑块处去乙酰化酶(SIRT1)和NADPH氧化酶P47亚基蛋白表达水平。结果 AS组比CON组GSH-Px、CAT、SOD活性均明显下降,MDA、ROS含量明显升高;沙棘黄酮干预能够改善以上指标;HE染色示血管内皮有所损伤;沙棘黄酮降低了AS大鼠NADPH氧化酶P47亚基的蛋白表达,升高了SIRT1蛋白表达水平。结论沙棘黄酮具有保护大鼠血管粥样硬化作用,提高机体防御氧化应激能力,可能与降低NADPH氧化酶蛋白表达及升高SIRT1蛋白表达有关。Objective To investigate whether TFH has improved the rats vascular function and its possible mechanism of atheroscle- rosis. Methods Fifty male SD rats were radomly divided into 5 groups： blank control group, atherosclerosis group, TFH low, medium and high dose group. After being fed for 60 days,rats in each group took aorta to detect vascular injury condition using HE staining. Gluta- thione peroxidase （ GSH - Px） , serum superoxide dismutase （SOD） and catalase （CAT） activity, malondialdehyde （MDA） content. Were determined Aorta was taken to determine ROS level by fluorescent probe. Acetylation enzyme （SIRT1） and NADPH oxidase subunit pro- tein P47 expression levels were detected by elisa technique. Results Compared AS group to CON,SOD, GSH - Px and CAT activity were significantly decreased, MDA,ROS content increased significantly. TFH intervention could improve the above indicators. Compared to AS group,TFH reduced NADPH oxidase P47, increased the protein expression of SIRT1 protein levels. Conclusion TFH has protective effects of vascular atherosclerosis. Its mechanism could be by lowering P47 expression and reducing ROS generation. There may be some sort of regulation action between SIRT1 and NADPH oxidase.

关 键 词：沙棘黄酮 动脉粥样硬化 P47 SIRT1 

分 类 号：R543[医药卫生—心血管疾病]