醒脑益智胶囊对亚硝酸钠致小鼠记忆巩固障碍模型的影响  

Effects of Xingnaoyizhi capsule on learning and memorizing consolidation of dysmnesia mice induced by sodium nitrite

作　　者：侯林[1] 李万忠[2] 张晓平[1] 聂克[1] 田景振[1] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]潍坊医学院,潍坊261053

出　　处：《中药药理与临床》2013年第6期116-118,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：重大新药创制专项-治疗老年性痴呆创新中药(2011ZX09102-003-06)

摘　　要：目的:研究醒脑益智胶囊对亚硝酸钠致小鼠记忆巩固障碍模型的影响。方法:小鼠60只随机分为空白对照组,模型组,石杉碱甲0.05 mg/kg,醒脑益智胶囊高剂量组、中剂量组、低剂量组(179.2,89.6,44.8 mg/kg),每天ig给药2次,连续15d进行预防性给药。给药后一次性皮下注射亚硝酸钠(120 mg/kg)复制小鼠学习记忆巩固障碍模型,避暗法、跳台法测定学习记忆能力,测定脑组织中超氧化物岐化酶(SOD)活力、丙二醛(MDA)的含量。结果:与模型组比较,醒脑益智胶囊中(89.6 mg/kg)、高剂量组(179.2 mg/kg)能在一定程度上延长小鼠潜伏期,减少5分钟内错误次数,提高SOD活力,降低MDA的含量,以高剂量治疗效果尤为明显。结论:醒脑益智胶囊有促进记忆和改善亚硝酸钠致小鼠记忆巩固障碍的作用。Objective： To study the effect of xingnaoyizhi capsule on learning and memorizing consolidation of dysmnesia mice induced by sodium nitrite（ NaNO2 ）. Methods： Sixty mice were divide into normal control group, model group, huperzine-A group（0.05 mg/kg ）, Xingnaoyizhi capsule of high dose group, middle dose group and low dose group（ 179.2 ,89.6,44.8 mg/kg）. After the intragastric administration twice a day respectively for 15 days, the learning and memory consolidation of dysmnesia model was induced by NAN02 （120 mg/kg）subcutaneous injection,different doses of Xingnaoyizhi capsule were orally administered to the mice respectively. Learning and memory function of various group rats were assessed with step-through test and step-down test; The content of malondiadehyde （ MDA）, activity of superexide dismutase （SOD） in brain tissue were detected. Results： Compared with model group, Xingnaoyizhi capsule（ 89.6 and 179.2 mg/kg for mice）pro- longed the latency periods of memory and significantly reduced the error times within 5 mins in dysmnesia groups （p 〈 0.05）, increased the activity of SOD and decreased the content of MDA in brain tissue. The high-dose therapy was specially obvious. Conclusions： Xingnaoyizhi capsule has protective effects on learning and memorizing consolidation of dysmnesia mice induced by sodium nitrite.

关 键 词：醒脑益智胶囊 学习记忆 超氧化物岐化酶 丙二醛 

分 类 号：R285[医药卫生—中药学]