ERK5信号通路介导流体剪切力对成骨细胞BMP2,BMP7mRNA的表达影响  

Fluid Shear Str ess Regulates BMP2/BMP4 mRNA Expression in MC3T3-E1 Osteoblastic Cells Via ERK5 Signaling Pathway

作　　者：滕元君[1] 赵良功[1] 陈少龙[1] 姜金[1] 崔兆辉[1] 夏亚一[1] 汪静[1] 王翠芳[1] 安丽萍[1] 马靖琳[1] 

机构地区：[1]兰州大学第二医院骨科,甘肃省骨关节疾病研究重点实验室,甘肃兰州730000

出　　处：《中国医学物理学杂志》2014年第1期4691-4693,4698,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81071478);甘肃省自然基金(1107RJZA 144);兰州大学第二医院院内课题(YJ2010-03)

摘　　要：目的:观察流体剪切力对MC3T3-E1成骨细胞BMP2,BMP7mRNA的表达影响,并探讨ERK5信号通路在其中的作用。方法:应用不同浓度的ERK5特异性阻断剂BIX02188干预成骨细胞,MTT比色法检测酶标仪490 nm吸光度,观察成骨细胞增殖情况以及ERK5mRNA的表达。并采用生理强度为12dyne/cm2的流体剪切力干预成骨细胞,实时荧光定量PCR检测BMP2,BMP7mRNA的表达情况。结果:浓度为15μM的BIX02188干预成骨细胞后,成骨细胞的生长的抑制以及ERK5mRNA的降低呈浓度依赖性。流体剪切力能够明显增加BMP2和BMP7mRNA的表达(P<0.05),且该种反应能够被ERK5信号通路阻断(P<0.05)。结论:ERK5信号通路调控流体剪切力对成骨细胞BMP2,BMP7mRNA的表达。Objective：This study aimed to investigate the effect of ERK5 signaling pathway on bone morphogenetic protein （BMP） BMP2/BMP4 mRNA expression induced by fluid shear stress on the MC3T3-E1 osteoblastic cells. Methods： Different concentration of BIX02188, a specific inhibitor of ERKS, was used to inhibit the expression of ERK5 mRNA. MTT assay and real-time PCR were performed to evaluate the cellular proliferation and ERK5/BMP2/BMP4 mRNA expression, respectively. Results： The expression of ERK5 mRNA was well blocked by BIX02188 at 15/zmmoFL （P〈0.05）, and osteoblastic proliferation was also inhabited by BIX02188 （P〈 0.05）. In addition, FSS siguifieanfly stimulated the expression ofBMP2/BMP4 mRNA （P〈 0.05）. However, ERK5 down-regulated BMP2/BMP4 mRNA expression induced by FSS （P〈 0.05）. Conclusions： ERK5 signaling pathway plays essential roles in the expression ofBMP2/BMP4 mRNA induced by fluid shear stress on the MC3T3-E1 osteoblastic cells.

关 键 词：流体剪切力 成骨细胞 ERK5信号通路 骨形成蛋白 

分 类 号：R687.1[医药卫生—骨科学]