新疆棉花枯萎病菌营养体亲和群及互补指数的研究  

Vegetative Compatibility Groups and Complementation Index of Fusarium oxysporum f.sp.vasinfectum in Cotton in Xinjiang

作　　者：王雪薇[1] 王新华[1] 喻宁莉[1] 孙文姬[2] 丁之铨[2] 

机构地区：[1]新疆农业大学农学分院,乌鲁木齐830052 [2]中国农科院植保所,北京100094

出　　处：《棉花学报》2000年第2期91-94,共4页Cotton Science

基　　金："九五"国家棉花科技攻关项目资助

摘　　要：从采自新疆 2 3个不同县 (市或团场 )的棉花枯萎病菌 ( Fusarium oxysporum f.sp.vasinfec-tum) 52个菌株上获得 785个不能利用硝酸盐的突变体 ( nit) ,其中 nitl 占 70 .57% ,nit M占1 9.75% ,nit3( 8)占 9.68%。将 52个菌株分别与已知的 4个 7号小种菌株、一个 3号小种菌株和一个 8号小种菌株的突变体按 nit1或 nit3( 8)配nit M的方式等距离配对 ,(各对菌株间的配组数为 1 1～ 1 54) ,进行营养体亲和性的测定 ,结果表明 ,52个待测菌株与 7号小种的 4个标准菌株间存在着不同程度的营养体亲和性 ,而与 3号小种和 8号小种的标准菌株均不亲和 ,因此 ,它们属于7号小种营养体亲和群—VCG70 1。但各菌株与 7号生理小种的 4个标准菌株间的互补指数存在着差异 。nitrate nonultilizing (nit) mutants were obtained from 52 strains of [WT5BX]Fusarium oxysporum [WT5BZ]f.sp.[WT5BX]vasinfectum,[WT5BZ]which were collected from 23 different counties (towns of farms) in Xinjing. Among them,70.57% were of nit1, 19.75% were of nitM, and 9.68% were of nit3(8). Being paired nit1 of nit3(8) with nitM between each of the strains and 4 known strains of race7, one known strain of race 3, and one known of race 8 at the same distance apart on MM medium, 52 strains were all compatible with at least one of the four strains of race 7, so they all belonged to the vegetative compatibility group of race 7-VCG701.However,neither of them was compatible with the strains of race3 or race8. The complementation index between each of 52 strains and the four strains of race 7 were different, which indicated the difference of individuals among one VCG in physiology and genetics.

关 键 词：新疆 棉花 枯萎病 菌株 亲和性 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]