骨关节炎软骨潮线漂移与软骨退变的相关性研究  

Correlation Study of Cartilage Tidal Line Drift and Degradation in Osteoarthritis

作　　者：李西海[1] 梁文娜[2] 叶蕻芝[1] 刘发元[1] 李会婷[1] 郑春松[1] 吴广文[1] 许惠凤[1] 陈文列[1] 刘献祥[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350122 [2]福建中医药大学中医学院中医证研究基地,福建福州350122

出　　处：《风湿病与关节炎》2014年第1期10-15,共6页Rheumatism and Arthritis

基　　金：国家自然科学基金(81102609);福建省自然科学基金(2011J05074);福建省科技厅重点项目(2012Y0046);福建省高校杰出青年科研人才计划(JA12165)

摘　　要：目的:探讨骨关节炎软骨潮线漂移与软骨退变的相关性。方法:选取8周龄SD大鼠20只,随机分为正常组和造模组,每组10只。采用4%木瓜蛋白酶双膝关节腔注射复制骨关节炎模型,8周后取双侧膝关节常规固定、脱钙、包埋和切片,分别采用苏木精-伊红、番红O-固绿、甲苯胺蓝和Masson三色染色法,观察骨关节的组织形态学变化。结果:正常关节面光滑平整,细胞排列整齐,四层结构清晰,潮线完整呈波伏浪起的曲线,软骨下骨小梁排列规律;骨关节炎关节面粗糙变形,细胞排列紊乱,四层结构不清晰,可见多重潮线或潮线中断,软骨下骨硬化或囊变。结论:软骨潮线漂移是骨关节炎软骨退变进程中必经的病理变化。Objective：To investigate the correlation between cartilage tidal line drift and degeneration in osteoarthritis.Methods：20 SD rats of 8 weeks old were randomly and equally divided into the normal group and the model group.Injections of 4% papain were given to their double knee joint cavities to replicate the osteoarthritis models.After 8 weeks,the bilateral knee joints of the rats were taken conventionally, fixed,decalcif ied,embedded and sliced,respectively using hematoxylin-eosin,safranin O-fast green,toluidine blue and Masson trichrome staining,observing the osteoarticular histological changes.Results：The normal joints characterized smooth articular surface,cells arranging in neat rows,four-layer structure being clear, tidal lines being complete wavy and subchondral bone trabeculas being regular; but the joints with osteoarthritis characterized deformed and rough articular surface,cells arranging in disorder, the four-layer structure being not clear,multiple tidal lines or interrupted tide lines being visible and subchondral bone trabeculas being sclerotic and cystic. Conclusion：Cartilage tidal line drift is the necessary pathological changes in the process of cartilage degeneration in osteoarthritis.

关 键 词：骨关节炎 软骨 潮线 软骨退变 软骨下骨 相关性 大鼠 

分 类 号：R684[医药卫生—骨科学]