乌药中异乌药内酯的含量考察  

Content determination of isolinderalactone in Radix linderae

作　　者：左美玲[1] 

机构地区：[1]湖南省长沙市第四医院,湖南长沙410006

出　　处：《中国现代医学杂志》2013年第33期58-60,共3页China Journal of Modern Medicine

摘　　要：目的建立用HPLC法测定乌药中异乌药内酯含量的方法。方法色谱柱为Kromasil Cl8柱(250 mm×4.6 mm,5μm),流动相:甲醇-乙腈-水(35∶25∶40 v/v);检测波长:235 nm;柱温:40℃;流速:1 mL/min。结果异乌药内酯的进样量在0.15μg^1.52μg(r=0.9997,n=6)的范围内与峰面积呈良好的线性关系,平均回收率为98.81%,RSD为1.52%。结论该法简单、可靠,可作为控制乌药药材质量的参考标准。【Objective】To establish a method for determination of isolinderalactone in radix linderae by RP- HPLC. 【Methods】The sample was separated on Kromasil Cl8（250 mm × 4.6 mm, 5 μm） column. The mobile phase was ethanol acetonitrile water（35：25：40 v/v） at a flow rate of 1.0 mL·min-1 and the detection wavelength was 235 nm and the column temperature was 40℃. 【Results】The calibration carves were linear in the range of 0.15 μg^1.52 μg（r =0.9997, n =6）. The average recoveries of isolinderalactone were 98.81%, RSD were 1.52%. 【Conclusion】The method is simple, accurate, reproducible and can offer referenees for the quality control of radix linderae.

关 键 词：乌药 异乌药内酯 高效液相色谱法 

分 类 号：R961.1[医药卫生—药理学]