水中呋喃丹的柱前衍生-高效液相色谱测定法  

Determination of carbofuran in water by high performance liquid chromatography with pre-column derivatization

作　　者：张明月[1] 李建平[1] 马永民[1] 渠志华[1] 

机构地区：[1]天津市疾病预防控制中心理化室,天津300011

出　　处：《环境与健康杂志》2013年第12期1106-1107,共2页Journal of Environment and Health

摘　　要：目的建立水中呋喃丹的柱前衍生一高效液相色谱测定法。方法水样经高效液相色谱柱前衍生，采用荧光检测器（激发光波长：339nm；发射光波长：445nm），色谱柱为DIKMAC18（150mm×4．6mm，5μm），流动相为甲醇-磷酸二氢钾（体积比为60：40），流量为1．0ml／min，柱温为室温。结果该方法标准曲线回归方程为y=49．995x-0．240，相关系数为0．9998，检出限为0．125μg／L。平均加标回收率为90．2％～94．5％，相对标准偏差（RSD）为3．98％-4．63％。结论该方法操作简便、快速，试剂用量小，适合于水中呋喃丹的含量检测。Objective To establish a high performance liquid chromatography method for the determination of carbofuran in water. Methods HPLC with pre-column derivatization, chromatographic column of DIKMA C18 （150 min×4.6 mm, 5μm） were used at room temperature and the mobile phase was methanol-potassium dihydrophospate （V：V=60：40） at a flow rate of 1.0 ml/min. Fluorescence detector wavelength was 339 nm （Ex） and 445 nm （Era）. Results The regression equation was y= 49.995x-0.240 with the correlation coefficient of 0.999 8. The detection limit was 0.125 μg/L. The average rates of recovery were 90.2%- 94.5% and the relative standard deviations were 3.98%-4.63%. Conclusion The method established in the present paper is applicable to the determination of carbofuran in water for the precision, accuracy and sensitivity,and less chemical regants.

关 键 词：色谱法 液相 农药 呋喃丹 柱前衍生 

分 类 号：O657.7[理学—分析化学]