提莫菲维小麦与光稃野燕麦易位系TGF_314的顺序C-分带-基因组原位杂交鉴定  

Sequential C-banding-GISH Identification of the Translocation Line TGF_314 fromTriticum timopheevi×Avena fatua L.var.glabrata Pat

作　　者：郑会敏[1] 王琳[1] 安洪周[1] 李鹏飞[1] 张素勤[1] 耿广东[1] 张庆勤[1] 

机构地区：[1]贵州大学南区农学院,贵阳550025

出　　处：《西北农业学报》2013年第12期21-24,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：贵州大学研究生创新基金(农科2012020);贵州大学自然科学专项科研基金(贵大专基合字(2013)01号);国家自然科学基金(31160224);科技部农业科技成果转化资金(2010GB2F200429);农业部计划项目(2011-A2521-520111-A0105-013);贵州省农业攻关项目(黔科合NY字[2008]3017;黔科合带帽字[2009]5002)

摘　　要：采用顺序C-带-基因组原位杂交(GISH)技术对提莫菲维小麦与光稃野燕麦远缘杂交后代中的外源遗传物质进行鉴定。GISH分析结果表明,在根尖细胞有丝分裂中期染色体具有2对清楚的杂交信号,说明该后代中可能含有稳定的外源遗传物质,且以易位的染色体片段形式存在。对该后代进行顺序C-带-GISH分析将杂交信号分别定位于提莫菲维小麦的7A和6G染色体短臂上,提莫菲维小麦的7A和6G染色体中含有光稃野燕麦的遗传物质。Sequential C-banding-GISH technique was applied to identified alien chromatins of the progenies from wide cross Triticum timopheevi X Arena fatua L. var. glabrata pat. GISH analysis showed that there were two pairs of clear signal sites at the metaphase of the root tip mitosis, illustra ting that stable alien chromatins were included in the progeny, and they were chromosome transloca tion. Sequential C-banding-GISH results showed that the alien chromatins from Arena fatua L. var. glabrata pat were located at the short arms of the chromosomes 7A and 6G.

关 键 词：小麦 野燕麦 C-带 GISH 鉴定 

分 类 号：S512.1[农业科学—作物学]