转过氧化氢酶基因KatG棉花的抗旱性  

Drought Resistance of KatGTransgenic Cotton

作　　者：蔡永智[1] 祝建波[1] 郝晓云[1] 王爱英[1] 徐登献[1] 

机构地区：[1]石河子大学生命科学学院,石河子大学农业生物技术重点实验室,新疆石河子832003

出　　处：《西北农业学报》2013年第12期56-61,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：转基因生物新品种培育重大专项课题"转基因棉花环境安全评价技术"(2011ZX08011-002)

摘　　要：以导入过氧化氢酶基因KatG的第3代转基因棉花为供试材料,通过大田试验,从卡那霉素检测出来的棉花植株中,经PCR筛选出阳性植株并进行抗旱性研究。结果显示,转基因棉花发芽率达86%以上,苗期叶绿素含量有显著增加;在苗期、蕾期和花期水分胁迫下,转KatG基因棉花在蕾期与花期的过氧化氢酶(CAT)活性显著增加;净光合速率(Pn)、气孔导度(Gs)、胞间CO2摩尔分数(Ci)和光系统Ⅱ最大光化学效率(Fv/Fm)显著高于对照;农艺性状的分析表明,转基因棉花抗旱性有明显提高;在干旱胁迫条件下,转KatG基因棉花表现出优良的生长和生理优势,可提高棉花抗旱能力。T3 generation of KatG transgenic upland cotton was applied to investigate the drought re sistance of transgenic cotton. The positive transgenic cotton plants were identified from the cotton plants with kanamycin resistance through PCR detection. The results proved that the germination rate of transgenic plants would be over 86%, and the chlorophyll content was significantly high in seed ling. Water stress resistance tests were carried out in seedling, budding and flowering stage respec tively. The contents of catalase（CAT）, NET photosynthetic rates （Pn）, stomatal conductance （G）, intercellular CO2 concentration （C） and maximum photochemical yield of PSⅡ （Fx/Fm） of KatG cot-ton were significantly higher than those of non-transgenic plants on the seedling, budding and flower ing stage. Analysis of agronomic traits showed that drought resistance of transgenic cotton was signif-icantly improved. These results demonstrated that te KatG gene expressed well in growth and devel- opment of the transgenie cotton plants, and indicated that the KatG transgenic cotton could improve the drought resistance.

关 键 词：棉花 过氧化氢酶基因 农艺性状 生理指标 抗旱性 

分 类 号：S562[农业科学—作物学]