纳米银溶胶与大肠杆菌作用及作用机理研究  

Study on the Process and Mechanism of Nano-silver Sol Against E.coli

作　　者：王静[1,2] 冀志江[2] 水中和[1] 赵春艳[2] 王晓燕[2] 曹延鑫[2] 

机构地区：[1]武汉理工大学材料科学与工程学院,武汉430070 [2]中国建筑材料科学研究总院绿色建筑材料国家重点实验室,北京100024

出　　处：《材料导报》2013年第24期4-8,21,共6页Materials Reports

基　　金：国家十二五科技支撑计划课题(2012BAJ02B08;2012BAB18B04-2-1;2012BAJ20B02-05)

摘　　要：选择纳米银溶胶材料样本,研究其与大肠杆菌作用的过程、效果和抗菌原理。扫描电子显微镜(SEM)观测显示溶胶液干燥后的颗粒呈球形,激光粒度仪分析发现其粒度D(0.9)为226nm;并用原子吸收法分析了Ag的溶出量。采用最小抑菌浓度(MIC)方法测试了其最小抑菌浓度值为0.2mg/mL;配制4种浓度纳米银溶胶悬浮液与大肠杆菌作用,测试不同时间OD600值,结果表明,质量浓度为0.2mg/mL以上的溶液OD600值都很低,且随时间延长呈下降趋势。对纳米银和大肠杆菌作用混合体进行定型,负染,用透射电镜(TEM)观测其切片前后形貌发现:纳米银处理后的大肠杆菌菌体模糊、破溃、胞内物质流出;切片样品发现了菌内空泡变性。分析认为纳米银溶胶有银溶出,其进入菌体内部,攻击细胞核质,使细菌细胞变性或破溃而死亡。Abstract The antimierobial activity and antibacterial mechanism of nano silver sol against t.. cotz was mves^l gated. The morphology of the desiccated nano silver sol was observed by scanning electric microscopy, and its particle size was analyzed by laser. The particle shape is round, and the size D（0. 9） was 226 nrn. The dissolubility of Ag in the nano siliver sol was investigated by Atomic Absorption Spectrometry （AAS）. The minimal inhibitory concentra- tion （MIC） of the nano silver sol against bacteria was 0. 2 mg/mL. Four different concentrations of the nano-silver sol were prepared to act with the E. coli, and the OD600 values varying with time were measured. The results confirmed that OI^oo values were very low and showed a downward trend over time, when the nano silver solution up to 0. 2 rag/ mL concentration. The mixture of nano-sliver sol and E. coli was solidified and negative stained, and then observed by transmission electron microscopy （TEM） before and after cutting into slices. The images showed that the treated E. coli cells were damaged and had intraeellular material flowed. The sections＇ images showed that the cytoplasm of the E. coli appeared vacuolar degeneration. It was suggested that the silver element dissolved into the solution, and then attacked nucleus and made vacuoles degeneration, resulting in death of the cell.

关 键 词：纳米银溶胶 大肠杆菌 抗菌 

分 类 号：TQ174[化学工程—陶瓷工业]