肝脂酶基因启动子-514C/T多态性与糖尿病慢性肾脏疾病的相关性研究  

Study of the association between hepatic lipase gene promoter region-514C/T polymorphism and chronic kidney disease in diabetes

作　　者：石柔[1] 刘华[2] 雷又鸣[3] 宋滇平[1] 陈波 向茜 

机构地区：[1]昆明医科大学第一附属医院糖尿病科,650032 [2]昆明医科大学第一附属医院检验科,650032 [3]昆明医科大学第一附属医院干疗科,650032 [4]云南省第三人民医院肾内科 [5]个旧市人民医院内分泌科

出　　处：《中国糖尿病杂志》2014年第1期12-15,共4页Chinese Journal of Diabetes

摘　　要：目的探讨肝脂酶(HL)基因启动子区-514C/T多态性与糖尿病慢性肾脏疾病(CKD)的相关性。方法纳入T2DM患者(T2DM组)164例,根据UAlb水平再分为CKD亚组(CKD,n=108)和无CKD亚组(CKD0,n=56);另选健康对照者(NC组)91名。运用限制性片段长度多态性(PCR-RFLP)技术对各组HL基因启动子区-514C/T多态性进行检测,并比较各组间基因型和等位基因频率及相关资料。结果 (1)CKD亚组C/T+T/T基因型和T等位基因频率高于CKD0亚组(P<0.05);(2)NC组与T2DM组间基因型和等位基因频率差异无统计学意义(P>0.05);(3)T2DM组中C/T+T/T基因型患者TG水平高于C/C基因型患者(P<0.05);(4)Logistic回归分析表明,HL基因启动子区-514C/T基因多态性与CKD相关。结论 HL基因启动子区-514T等位基因可能是CKD发生的遗传易感因素。Objective To investigate the association between hepatic lipase（HL）gene promoter region-514C/T polymorphism and chronic kidney disease （CKD）in diabetes. Methods The polymorphism of HL gene promoter-514C/T in 164 patients with T2DM included pure T2DM （CKD0, n=56） group,T2DM patients with CKD（CKD group, n= 108） and 91 normal controls（NC group）were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. The genotype frequency and allele frequency and relative clinical data were compared among groups. Results （1） The C/T＋T/T genotype and T allele frequencies were significantly higher in CKD group than in CKD0 group （P〉0.05）;（2）There were no significant differences in genotype frequencies and allele frequencies between NC and T2DM （CKDO ＋ CKD） group （P〉 0.05） ; （3） In T2DM group, triglyceride （TG） was significantly higher in C/T＋ T/T genotype group than in C/C genotype group （P〈 0.05）; （4） Logistic regression analysis showed that HL gene promoter region 514C/T polymorphism were significantly related to CKD. Conclusion The HL 514T allele may be the genetic predisposing factor for CKD development.

关 键 词：肝脂酶基因 基因多态性 糖尿病慢性肾脏疾病 

分 类 号：R587.2[医药卫生—内分泌]