BMP-7对大鼠脑缺血再灌注损伤后Caspase-9表达的影响  

Effect of bone morphogenetic protein-7 on expression of Caspase-9 and Caspase-3 in rats with focal cerebral ischemia-reperfusion

作　　者：裴海涛[1] 曹东明[1] 李红云[1] 郭壮丽[1] 

机构地区：[1]青岛大学医学院附属医院急诊神经科,山东青岛266003

出　　处：《中风与神经疾病杂志》2014年第1期18-21,共4页Journal of Apoplexy and Nervous Diseases

基　　金：山东省自然科学基金资助项目(No ZR2009CM121)

摘　　要：目的观察骨形态发生蛋白-7(BMP-7)对大鼠脑缺血再灌注损伤后皮质Caspase-9表达的影响,探讨其神经保护机制。方法成年雄性Wistar大鼠40只,随机分为假手术组、模型组、BMP-7治疗组(0.1 mg/kg,0.2 mg/kg),每组10只,模型组和BMP-7治疗组采用线栓法制作大脑中动脉缺血再灌注模型,Longa评分法进行神经功能评分,HE染色观察缺血侧皮质病理学变化,实时定量RT-PCR法检测Caspase-9 mRNA的表达,免疫组织化学法及Western blot法检测Caspase-9蛋白的表达。结果与模型组相比,BMP-7治疗组大鼠神经功能评分明显降低(t=3.79,t=4.43,P<0.01),HE染色显示缺血侧皮质脑组织病理损伤减轻,且以高剂量组较为明显,实时定量PCR显示Caspase-9 mRNA表达明显降低(t=2.56,t=4.52;P<0.05,P<0.01),Western blot检测显示Caspase-9蛋白表达明显减少(t=3.11,t=5.62;P<0.05,P<0.01)。结论 BMP-7能减轻缺血再灌注损伤脑组织损伤程度,其机制可能与下调线粒体凋亡途径中Caspase-9的表达有关,而且保护作用与剂量呈正相关。Objective To study the neuroprotective effect and mechanism of bone morphogenetic protein-7 on ex- pression of Caspase-9 after focal cerebral ischemia-reperfusion injury in rats. Methods Total of 40 male Wistar rats were randomly divided into five groups ： sham-operated group, model group, BMP-7 injection group （ 0.2mg/kg ,0.1 mg/kg as high and low dose respectively） consisting of 10 rats each group. Focal cerebral ischemie model was established by inserting a monofilament thread into the middle cerebral artery. Neurological functuion deficits were evaluated by Longa test scale. The neuronal pathological change in isehemic eorex was observed by HE staining. The expressions of caspase-9 mRNA and its protein in ischemia cortex were determined by Real-time PCR, immunohistochemistry and Western blot respectively. Re- suits As compared with model group, the pathological damage in the ischemia cortex was significantly lessened and most significantly in high dose group, the neurological deficiency scores were lower（ t = 3.79, t = 4.43, P 〈 0.01 ）, the expression of Caspase-9 mRNA was lower（t = 2.56, t = 4.52 ; P 〈 0.05, P 〈 0.01 ） and Caspase-9 protein decreased （ t = 3.11, t = 5.62 ; P 〈 0.05, P 〈 0. 01 ） significantly than those in BMP-7 treatment group. Conclusion BMP-7 could protect focal cer- ebral isehemia reperfusion injury by inhibiting the expression of Caspase-9 in mitochondrial apoptotie pathway as dose-de- pendently.

关 键 词：BMP-7 脑缺血 再灌注损伤 细胞凋亡 CASPASE-9 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]