PRRSV特异性肽对CSF、PRRS免疫猪IFN-γ、IL-10分泌的影响  

Effect of PRRSV-specific peptide on secretion of IFN-γ and IL-10 after CSF and PRRS vaccination

作　　者：郎广平[1] 郭敏[1] 梁志选[2] 冯娇[1] 孔惟博 任少敏[1] 李彬[1] 韩盈盈[1] 赵建增 高英杰[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]天津市动物疫病预防控制中心,天津300402 [3]华威特(北京)生物科技有限公司,北京100085

出　　处：《中国兽医学报》2014年第2期187-191,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972193)

摘　　要：应用PRRSV-GP5特异性肽刺激CSF、PRRS免疫猪外周血淋巴细胞,采用ELISA方法测定不同时间点淋巴细胞分泌IFN-γ及IL-10的水平,分析PRRSV-GP5特异性肽刺激后,淋巴细胞培养上清中细胞因子的变化规律。结果显示,CSFV高抗PRRSV低抗体组于PRRSV免疫14d,肽刺激组中IFN-γ水平显著高于对照组(P<0.05);其他组均差异不显著(P>0.05)。PRRSV免疫14d,细胞培养24,72h后加PRRSV-GP5肽刺激,各组中试验组与对照组相比IL-10水平明显降低(P<0.01);PRRSV免疫28d,各组中肽刺激组与空白组相比,IL-10水平均无显著性差异(P>0.05)。结果表明,PRRSV-GP5肽在PRRSV免疫早期可以促进IFN-γ的分泌,并抑制IL-10的产生,但在免疫后期对淋巴细胞分泌的调节作用还需进一步研究。The levels of IFN-7 and IL-10 secreted by lymphocytes stimulated with PRRSV-GP5- specific peptide were measured by ELISA at different time points. And changes of eytokines in lymphocyte culture supernatant were analyzed. The results showed that in high-CSFV-low-PRRSV antibody group in the level of IFN-7 was significantly higher than that of peptide-stimulated group and the control group at 14 d after PRRSV vaccination （P〈0. 05） ;other groups were not sigifi cant difference （P〉0. 05）. At 14 d after PRRSV vaccination,the concentration of IL-10 in peptide stimulated group was significantly lower than that in the control group which were cultured for 24 h and 72 h （P〈0.01）;there was no significant difference in the level of IL-10 compared with the peptide-stimulated group and the control group at 28 d after PRRSV vaccination（P〉0.05）. The results indicated that PRRSV-GP5-specific peptide could promote the secretion of IFN-7 and inhibits the production of IL-10 at the early time of PRRSV vaccination,but still need further stud- y on the immunity of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 肽 ELISA IFN-Y IL-10 

分 类 号：S852.65[农业科学—基础兽医学]