温室栽培砂糖橘花粉活力测定方法比较研究  

Comparison Study on Pollen Viability Determining Methods of Citrus reticulate 'Shatangju' in Solar Greenhouse

作　　者：高兴颖[1] 崔世茂[1] 付崇毅[2] 刘微[1] 褚义红[1] 朱山川[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]内蒙古农牧业科学院蔬菜研究所,内蒙古呼和浩特010031

出　　处：《北方园艺》2014年第3期39-41,共3页Northern Horticulture

基　　金：内蒙古科技厅科技推广资助项目(20110710)

摘　　要：以5a生枳砧砂糖橘嫁接苗为试材,研究比较了离体萌发法、TTC染色法、I2-KI染色法和MTT染色法对花粉活力的测定效果及其对砂糖橘有叶花和无叶花2种类型离体花粉活性的影响。结果表明:砂糖橘无叶花花粉活性高于有叶花花粉活性;MTT染色法是简单快速测定砂糖橘花粉活性的最佳方法。Taking grafted seedings of Citrus reticulate ＇Shatangju＇ on rootstock of trifoliate orange as material, the determinauon effects of I2-KI,TTC.MTT and zn wtro pollen germination method were compared and their effect on in vitro activity of pollen between the different types of flower（the flower with leaves and non-leaf flower） of Citrus reticulate ＇Shatangju＇ were studied. The results indicated that the pollen of Citrus reticulate ＇Shatangiu＇ viability of non- leaf flower .was higher than the pollen viability of flower with leaves. MTT staining method was the optimum and rapid staining method to determine pollen viability of Citrus reticulate ＇Shatangju＇.

关 键 词：砂糖橘 花粉活力 离体萌发法MTT染色法 TTC染色法 I2-KI染色法 

分 类 号：S666.2[农业科学—果树学]