矮壮素处理对青花菜生长状态及春化关键酶活性的影响  

Effects of CCC treatment on vernalization-key enzyme activity in broccoli

作　　者：吴美璇[1] 郑姗姗 蒋欣梅[1] 于锡宏[1] 柳杨[1] 车欣[1] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]肇州县农业局,黑龙江大庆166400

出　　处：《浙江大学学报（理学版）》2014年第1期63-66,共4页Journal of Zhejiang University（Science Edition）

基　　金：黑龙江省自然科学基金资助项目(C201039)

摘　　要：以中早熟品种‘元绿701’青花菜为试材,当植株长至3片真叶时叶面喷施不同浓度(2 000、3 000、4 000mg·L-1)的矮壮素溶液,在其长到6片真叶时进行低温春化处理(昼温/夜温=(13.3±0.5)℃/(6.3±0.5)℃),研究矮壮素处理对青花菜生长状态及春化相关酶活性的影响.结果表明:喷施矮壮素后,改变了植株的营养生长状态,使株高显著降低、茎粗显著增加;降低了转化酶和硝酸还原酶的活性,提高了谷氨酰胺合成酶活性.低水平的转化酶、硝酸还原酶、谷氨酰胺合成酶活性有利于春化的完成.Using middle-early variety ＂yuan-lti 701＂ broccoli as the material, the effects of CCC on growing and ver- nalization-related enzyme activity by spraying different concentration（2 000, 3 000, 4 000 mg ~ L 1） of CCC when the plants had three leaves and doing low temperature vernalization treatment when the plants had six leaves in broc- coli are researched. The results showed that, vegetative growth of broccoli plants had been changed, which plant height was reduced and stem diameter was increased significantly, and the completion of vernalization had been ac- celerated under CCC treatment. The Inv activity and NR activity were reduced while GS activity was increased. In addition, low level Inv activity,NR activity and GS activity could promote completion of vernalization.

关 键 词：青花菜 矮壮素 春化 酶活性 

分 类 号：S63[农业科学—蔬菜学]