噬菌弧菌H2的分离鉴定与噬菌活性分析  

Isolation,identification of Bacteriovorax sp.H2 and its lysis activity analysis

作　　者：侯三玲[1] 曹海鹏[1] 李洋 何珊[1] 胡鲲[1] 杨先乐[1] 

机构地区：[1]上海海洋大学水产与生命学院、国家水生动物病原库,上海201306 [2]上海市闵行第五中学,上海200240

出　　处：《环境污染与防治》2014年第1期19-23,27,共6页Environmental Pollution & Control

基　　金：国家“863计划”项目(No.2011AA10A216);国家重大科技成果转化项目(No.ZD-2012-345-5);公益性行业(农业)科技专项资金资助项目(No.201203085);现代农业产业技术体系建设专项资金资助项目(No.CARS-46-12);江苏省农业科技支撑计划项目(No.BE2013366);山西省科技攻关项目(No.20120311025-4);上海高校实验技术队伍建设计划项目

摘　　要：以霍乱弧菌作为宿主菌，从南美白对虾养殖水体中分离到一株具有噬菌特性的菌株H2，通过对其形态观察、特异性聚合酶链式反应（PCR）鉴定与系统发育分析，证实菌株H2为噬菌弧菌（Bacteriovoraxsp．）（GenBank登录号：JXl22409），其16SrRNA序列与GenBank基因库中噬菌弧菌属菌株的16SrRNA序列有94％～98％的同源性，而且与噬菌弧菌菌株NE1（GenBank登录号：EF092445）的亲缘关系最近。此外，菌株H2对溶藻弧菌、哈维氏弧菌、创伤弧菌、副溶血弧菌、鳗弧菌等其他弧菌也具有良好的噬菌活性，在pH=7．5、亚硝酸盐氮为0．2mg／L时具有良好的噬菌活性，在氨氮≥0．2mg／L时的噬菌活性降低。确定了噬菌弧菌H2的分类地位，分析了噬菌弧菌H2对常见弧菌的噬菌效果以及水环境因子对其噬菌活性的影响，不仅丰富了弧菌寄生菌的微生物资源，而且为其在养殖水体弧菌消除中的应用奠定了基础。A predatory bacterial strain H2 was isolated from the Litopenaeus vannamei farming water using Vibrio cholerae as the host bacterium. Strain H2 was identified as a Bacteriovorax sp. strain （GenBank accession No. JX122409） through morphology observation, specific PCR detection and phylogenetic analysis. The 16S rRNA se- quence of strain H2 had 94%-98% of homology with those of Bacteriovorax strains in GenBank,and was most close to Bacteriovorax strain NE1 （GenBank accession No. EF092445）. In addition,strain H2 could lyze the other bacterial pathogens such as Vibrio alginolyticus , Vibrio harveyi , Vibrio vulni ficus , Vibrio parahaemolyticus and Vibrio an- guillarum ,and exhibited good lytic activity under pH 7.5 and 0.2 mg/L nitrite nitrogen. However,its lysis activity could be inhibited at ≥0.2 mg/L ammonia nitrogen. The present study determined the taxonomic position of strain H2,analyzed its lysis effects on well-known Vibrio, which not only enrich the predatory microbial resources against Vibrio,but laid a solid basis for its application in the removal of Vibrio in farming waters.

关 键 词：弧菌 噬菌弧菌 鉴定 系统发育分析 噬菌活性 

分 类 号：S941.4[农业科学—水产养殖]